MCAM/CD146 Antibody (OTI5C4)

Novus Biologicals | Catalog # NBP2-45770

Novus Biologicals

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI5C4
View all MCAM/CD146 Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human MCAM (NP_006491) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

71.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Mouse MCAM/CD146 Antibody (OTI5C4) (NBP2-45770) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for MCAM/CD146 Antibody (OTI5C4)

Western Blot: MCAM/CD146 Antibody (OTI5C4) [NBP2-45770]

Western Blot: MCAM/CD146 Antibody (OTI5C4) [NBP2-45770]

Western Blot: MCAM/CD146 Antibody (5C4) [NBP2-45770] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MCAM.
Immunohistochemistry: MCAM/CD146 Antibody (OTI5C4) [NBP2-45770]

Immunohistochemistry: MCAM/CD146 Antibody (OTI5C4) [NBP2-45770]

Immunohistochemistry: MCAM/CD146 Antibody (5C4) [NBP2-45770] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min)
Immunohistochemistry: MCAM/CD146 Antibody (OTI5C4) [NBP2-45770]

Immunohistochemistry: MCAM/CD146 Antibody (OTI5C4) [NBP2-45770]

Immunohistochemistry: MCAM/CD146 Antibody (5C4) [NBP2-45770] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min)
Immunohistochemistry: MCAM/CD146 Antibody (OTI5C4) [NBP2-45770]

Immunohistochemistry: MCAM/CD146 Antibody (OTI5C4) [NBP2-45770]

Immunohistochemistry: MCAM/CD146 Antibody (5C4) [NBP2-45770] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min)
Immunohistochemistry: MCAM/CD146 Antibody (OTI5C4) [NBP2-45770]

Immunohistochemistry: MCAM/CD146 Antibody (OTI5C4) [NBP2-45770]

Immunohistochemistry: MCAM/CD146 Antibody (5C4) [NBP2-45770] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min)
Immunohistochemistry: MCAM/CD146 Antibody (OTI5C4) [NBP2-45770]

Immunohistochemistry: MCAM/CD146 Antibody (OTI5C4) [NBP2-45770]

Immunohistochemistry: MCAM/CD146 Antibody (5C4) [NBP2-45770] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min)
Immunohistochemistry: MCAM/CD146 Antibody (OTI5C4) [NBP2-45770]

Immunohistochemistry: MCAM/CD146 Antibody (OTI5C4) [NBP2-45770]

Immunohistochemistry: MCAM/CD146 Antibody (5C4) [NBP2-45770] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min)
MCAM/CD146 Antibody (OTI5C4)

MCAM/CD146 Antibody (OTI5C4)[NBP2-45770]

MCAM/CD146 Antibody (OTI5C4)[NBP2-45770]Simple Western� analysis of endogenous protein SMPD1 from HepG2 lysates (0.2 mg/mL) using SMPD1 Mouse Monoclonal Antibody. The virtual lane view (left) shows the target (as indicated) at 1:50 dilution of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at a 1:50 dilution of primary antibody. This experiment was performed under reducing conditions on the Jess� Simple Western instrument from ProteinSimple, a Bio-Techne brand, using the 12�230 kDa Separation Module.

Applications for MCAM/CD146 Antibody (OTI5C4)

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MCAM/CD146

CD146 belongs to the immunoglobulin supergene family with five immunoglobulin like domains (V-V-C2-C2-C2), a transmembrane region and a 63 residue cytoplasmic tail. The protein is a membrane glycoprotein that functions as a Ca2+ independent cell adhesion molecule involved in heterophilic cell to cell interactions. CD146 has a molecular size of 130 kDa in its reduced form (118 kDa unreduced), and N linked glycosylation accounts for fifty percent of the apparent molecular weight. In some cells the molecule carries a sulfate 3 glucuronyl moiety. Expression of the molecule was shown in a relatively limited spectrum of normal human tissues (endothelium, smooth muscle, and subpopulations of activated T cells) and malignant neoplasm (melanoma and breast carcinoma). The lineage specific expression pattern of CD146 can be useful in the differential diagnosis of certain lesions including melanomas and various types of gestational trophoblastic lesions. CD146 expression can promote tumor progression in human melanoma, through enhanced interaction between melanoma cells and endothelial cells. However, in breast carcinoma, CD146 may act as a tumor suppressor. Over expression of CD146 in breast carcinoma cells results in a more cohesive cell growth and in the formation of smaller tumors in nude mice. During implantation and placentation, CD146 is expressed by the intermediate trophoblast in the placental site and binds to its putative receptor in uterine smooth muscle cells thus limiting trophoblastic invasion in the myometrium. Monoclonal antibody specific for CD146 is an important tool for the identification and isolation of cells expressing CD146.

Long Name

Melanoma Cell Adhesion Molecule

Alternate Names

CD146, L-Gicerin, MUC18

Gene Symbol

MCAM

Additional MCAM/CD146 Products

Product Documents for MCAM/CD146 Antibody (OTI5C4)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for MCAM/CD146 Antibody (OTI5C4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for MCAM/CD146 Antibody (OTI5C4)

There are currently no reviews for this product. Be the first to review MCAM/CD146 Antibody (OTI5C4) and earn rewards!

Have you used MCAM/CD146 Antibody (OTI5C4)?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies