mCherry Antibody

Novus Biologicals | Catalog # NBP2-43720

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Non-species specific

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry Whole-Mount, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Proximity Ligation Assay

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all mCherry Primary Antibodies »

Product Specifications

Immunogen

Full length mCherry recombinant protein

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

27 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit mCherry Antibody (NBP2-43720) is a polyclonal antibody validated for use in IHC, WB, ICC/IF and IP. Anti-mCherry Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for mCherry Antibody

mCherry Antibody

Western Blot: mCherry Antibody [NBP2-43720] -

Western Blot: mCherry Antibody [NBP2-43720] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with mCherry antibody (NBP2-43720) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunoprecipitation: mCherry Antibody [NBP2-43720]

Immunoprecipitation: mCherry Antibody [NBP2-43720]

Immunoprecipitation: mCherry Antibody [NBP2-43720] - Analysis of 293T whole cell lysate/extractA : 20 ug whole cell lysate/extract of mcherry protein expressing 293T cellsB : Control with 2.5 ug of pre-immune rabbit IgGC : Immunoprecipitation of mcherry protein by 2.5 ug of mcherry antibody 7.5% SDS-PAGE The immunoprecipitated mcherry protein was detected by mcherry antibody diluted at 1 : 3000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.
Western Blot: mCherry Antibody [NBP2-43720]

Western Blot: mCherry Antibody [NBP2-43720]

Western Blot: mCherry Antibody [NBP2-43720] - Analysis of A. 5ng recombinant mCherry B. 10ng recombinant mCherry C. 20ng recombinant mCherry 12 % SDS-PAGE mCherry antibody dilution: 1:2000.
mCherry Antibody

Western Blot: mCherry Antibody [NBP2-43720] -

Western Blot: mCherry Antibody [NBP2-43720] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by gradient gel% SDS-PAGE, and the membrane was blotted with mCherry antibody (NBP2-43720) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
mCherry Antibody

Immunocytochemistry/ Immunofluorescence: mCherry Antibody [NBP2-43720] -

mCherry antibody detects mCherry protein at mCherry fusion protein by immunofluorescent analysis. br>Sample: mCherry fusion protein-transfected HeLa cells were fixed in 4% paraformaldehyde at RT for 30 min, then Triton X-100 for 1 min. br>Green: mCherry protein stained by mCherry antibody (NBP2-43720) diluted at 1:500. br>Red: mCherry is expressed in the transfected cell. Blue: Hoechst 33342 staining.
mCherry Antibody

Immunohistochemistry: mCherry Antibody [NBP2-43720] -

mCherry antibody labels mCherry-expressing cells on zebrafish embryos by whole mount immunohistochemical analysis.
Sample: 2 days-post-fertilization zebrafish embryo.
mCherry antibody labels mCherry-expressing cells (NBP2-43720) dilution: 1:100.

Applications for mCherry Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:500

Immunohistochemistry Whole-Mount

1:100-1:500

Immunoprecipitation

1:100-1:500

Western Blot

1:1000-1:10000
Application Notes
PLA, IHC-FR-Assay dependent

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: mCherry

mCherry is a monomeric red fluorescent protein (mRFP) belonging to the mFruits family which is brighter and more photostable compared to the first-generation mRFP1, making them ideal for fluorescence microscopy (1). mCherry has an excitation maximum at 587 nm and an emission maximum at 610 nm. mCherry protein was derived from DsRed, a red fluorescent protein from the coral Discosoma (disc anemone) (2). The red chromophore of DsRed has a similar topology to GFP, the green fluorescent protein isolated from the jellyfish Aequorea Victoria, but has extended pi-electron conjugation resulting in red-shifted absorbance and emission (3). mCherry is 236 amino acids (aa) in length with a theoretical molecular weight of 28 kDa and has a crystal structure with the chromophore forming a central helix shielded within an eleven-stranded beta-barrel (3).

mCherry can be used as a long-wavelength hetero-FRET (fluorescence resonance energy transfer) acceptor and probe for homoFRET experiments given its high peak molar absorptivity, folding efficiency, and superior spectral properties (4). Additionally, because mCherry does not interfere with other plasmids or alter the growth of Legionella species during intracellular growth, it can be used for constitutive gene expression in a variety of gram-negative bacterial species (5). For example, a plasmid developed to constitutively express mCherry under the Ptac promoter has been used in several Legionella species including L. pneumophila, the causative agent of Legionnaires' disease (5).

References

1. Shaner, N. C., Steinbach, P. A., & Tsien, R. Y. (2005). A guide to choosing fluorescent proteins. Nature Methods, 2(12), 905-909. doi:10.1038/nmeth819

2. Bevis, B. J., & Glick, B. S. (2002). Rapidly maturing variants of the Discosoma red fluorescent protein (DsRed). Nature Biotechnology, 20(1), 83-87. https://doi.org/10.1038/nbt0102-83

3. Wall, M. A., Socolich, M., & Ranganathan, R. (2000). The structural basis for red fluorescence in the tetrameric GFP homolog DsRed. Nature Structural Biology, 7(12), 1133-1138. https://doi.org/10.1038/81992

4. Akrap, N., Seidel, T., & Barisas, B. G. (2010). Forster distances for fluorescence resonant energy transfer between mCherry and other visible fluorescent proteins. Analytical Biochemistry, 402(1), 105-106. https://doi.org/10.1016/j.ab.2010.03.026

5. Gebhardt, M. J., Jacobson, R. K., & Shuman, H. A. (2017). Seeing red; the development of pON.mCherry, a broad-host range constitutive expression plasmid for Gram-negative bacteria. Plos One, 12(3), e0173116. https://doi.org/10.1371/journal.pone.0173116

Long Name

mCherry

Alternate Names

DSRED, red fluorescent protein mCherry, Red Fluoroscent Protein

Additional mCherry Products

Product Documents for mCherry Antibody

Certificate of Analysis

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Product Specific Notices for mCherry Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for mCherry Antibody

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for mCherry Antibody

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    • Q: Does this antibody cross-react with GFP epitopes? As I would like to use both GFP and mCherry antibodies during histochemistry I would not like them to cross-react.
      A: mCherry and GFP share just 29% sequence similarity, so this antibody is not predicted to cross-react to GFP and has never shown any ability to detect GFP in testing.
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