Melanoma Marker (MART-1 + gp100) Antibody (DT101+ BC199 + HMB45) - IHC-Prediluted
Novus Biologicals | Catalog # NBP2-48232
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2b Kappa/IgG1 Kappa Clone # DT101+ BC199 + HMB45
Format
IHC-Prediluted
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Product Specifications
Immunogen
Recombinant Melanoma Marker (MART-1 + gp100) protein (DT101 & BC199); Extract of pigmented melanoma metastases from lymph nodes (HMB45)
Localization
Cytoplasmic
Marker
Melanoma Marker
Specificity
This antibody cocktail recognizes two melanoma-specific proteins, which include MART-1 and gp100. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Function of gp100 is not known but it is reported to be a useful marker for melanocytes and melanomas. A cocktail of these two markers is sensitive for labeling formalin-fixed, paraffin-embedded melanomas and other tumors showing melanocytic differentiation.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa/IgG1 Kappa
Description
The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.
Scientific Data Images
![Immunohistochemistry-Paraffin: Melanoma Marker (MART-1 + gp100) Antibody (DT101+ BC199 + HMB45) - IHC-Prediluted [NBP2-48232]](https://resources.rndsystems.com/images/products/Melanoma-Marker-MART-1-+-gp100-Antibody-DT101+-BC199-+-HMB45-IHC-Prediluted-Immunohistochemistry-Paraffin-NBP2-48232-img0002.jpg "Immunohistochemistry-Paraffin: Melanoma Marker (MART-1 + gp100) Antibody (DT101+ BC199 + HMB45) - IHC-Prediluted [NBP2-48232]")
Immunohistochemistry-Paraffin: Melanoma Marker (MART-1 + gp100) Antibody (DT101+ BC199 + HMB45) - IHC-Prediluted [NBP2-48232]
Immunohistochemistry-Paraffin: Melanoma Marker (MART-1 + gp100) Antibody (DT101+ BC199 + HMB45) - IHC-Prediluted [NBP2-48232] - Formalin-fixed, paraffin-embedded human Melanoma stained with Melanoma Marker Mouse Monoclonal Antibody (DT101+ BC199 + HMB45).![Immunohistochemistry-Paraffin: Melanoma Marker (MART-1 + gp100) Antibody (DT101+ BC199 + HMB45) - IHC-Prediluted [NBP2-48232]](https://resources.rndsystems.com/images/products/Melanoma-Marker-MART-1-+-gp100-Antibody-DT101+-BC199-+-HMB45-IHC-Prediluted-Immunohistochemistry-Paraffin-NBP2-48232-img0001.jpg "Immunohistochemistry-Paraffin: Melanoma Marker (MART-1 + gp100) Antibody (DT101+ BC199 + HMB45) - IHC-Prediluted [NBP2-48232]")
Immunohistochemistry-Paraffin: Melanoma Marker (MART-1 + gp100) Antibody (DT101+ BC199 + HMB45) - IHC-Prediluted [NBP2-48232]
Immunohistochemistry-Paraffin: Melanoma Marker (MART-1 + gp100) Antibody (DT101+ BC199 + HMB45) - IHC-Prediluted [NBP2-48232] - Formalin-fixed, paraffin-embedded human Melanoma stained with Melanoma Marker Mouse Monoclonal Antibody (DT101+ BC199 + HMB45).Applications
Application
Recommended Usage
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Format
IHC-Prediluted
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Melanoma Marker (MART-1 + gp100)
Alternate Names
MART1, MART-1
Gene Symbol
MLANA
Additional Melanoma Marker (MART-1 + gp100) Products
Product Documents
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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