MLKL Antibody (SA40-04)
Novus Biologicals | Catalog # NBP2-67299
Recombinant Monoclonal Antibody
![Western Blot: MLKL Antibody (SA40-04) [NBP2-67299]](https://resources.rndsystems.com/images/products/MLKL-Antibody-SA40-04-Western-Blot-NBP2-67299-img0002.jpg "Western Blot: MLKL Antibody (SA40-04) [NBP2-67299]")
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Key Product Details
Species Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # SA40-04 expressed in HEK293
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Product Specifications
Immunogen
Recombinant protein within Human MLKL aa 333-471 / 471. (SwissProt: Q8NB16 Human)
Localization
Cytoplasm, Cell membrane, Golgi apparatus.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for MLKL Antibody (SA40-04)
Western Blot: MLKL Antibody (SA40-04) [NBP2-67299]
Western Blot: MLKL Antibody (SA40-04) [NBP2-67299] - Analysis of MLKL on different cell lysates using anti-MLKL antibody at 1/1,000 dilution. Positive control: Lane 1: HT29 Lane 2: HUVEC Lane 3: Hela![Immunohistochemistry-Paraffin: MLKL Antibody (SA40-04) [NBP2-67299]](https://resources.rndsystems.com/images/products/MLKL-Antibody-SA40-04-Immunohistochemistry-Paraffin-NBP2-67299-img0001.jpg "Immunohistochemistry-Paraffin: MLKL Antibody (SA40-04) [NBP2-67299]")
Immunohistochemistry-Paraffin: MLKL Antibody (SA40-04) [NBP2-67299]
Immunohistochemistry-Paraffin: MLKL Antibody (SA40-04) [NBP2-67299] - Analysis of paraffin-embedded human tonsil tissue using anti-MLKL antibody. Counter stained with hematoxylin.![MLKL Antibody (SA40-04) Western Blot: MLKL Antibody (SA40-04) [NBP2-67299]](https://resources.rndsystems.com/images/products/nbp2-67299_-western-blot-639204586671437426.jpg "Western Blot: MLKL Antibody (SA40-04) [NBP2-67299]")
Western Blot: MLKL Antibody (SA40-04) [NBP2-67299]
Western blot analysis on different lysates with NBP2-67299 at 1/5,000 dilution and competitor's antibody at 1/1,000 dilution. Lane 1: HUVEC cell lysate (20 µg/Lane) Lane 2: HT-29 cell lysate (20 µg/Lane) Lane 3: HeLa cell lysate (20 µg/Lane) Lane 4: THP-1 cell lysate (20 µg/Lane) Lane 5: U-937 cell lysate (20 µg/Lane) Lane 6: K-562 cell lysate (low expression) (20 µg/Lane) Lane 7: NIH/3T3 cell lysate (20 µg/Lane) Predicted band size: 54 kDa Observed band size: 54 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP2-67299 at 1/5,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.Applications for MLKL Antibody (SA40-04)
Application
Recommended Usage
Immunohistochemistry-Paraffin
1:50-1:200
Western Blot
1:1000-1:5000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
TBS (pH7.4), 0.05% BSA, 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: MLKL
Long Name
Mixed Lineage Kinase Domain-like
Alternate Names
FLJ34389, mixed lineage kinase domain-like, mixed lineage kinase domain-like protein
Gene Symbol
MLKL
Additional MLKL Products
Product Documents for MLKL Antibody (SA40-04)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for MLKL Antibody (SA40-04)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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