M.O.M.(R) (Mouse-On-Mouse) Elite(R) IgG HRP Immunodetection Kit

Novus Biologicals | Catalog # PK-2200-NB

Novus Biologicals
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Key Product Details

Species

Mouse

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Kit Type

HRP Immunodetection Kit

Product Summary for M.O.M.(R) (Mouse-On-Mouse) Elite(R) IgG HRP Immunodetection Kit

Features:
Significant reduction of endogenous mouse Ig staining when using mouse primary antibodies on mouse tissue
Clear, crisp specific staining of antigens of interest
Based on ImmPRESS(R) reagent protocols
Procedures are simple and easy to follow
No tedious calculations or antibody prebinding steps required

Product Specifications

Application Notes

The Vector M.O.M.(R) (Mouse-On-Mouse) Elite(R) IgG Immunodetection Kit contains enough stock reagents to produce about 25 ml of working solution which is generally sufficient to stain approximately 250 tissue section.

Scientific Data Images for M.O.M.(R) (Mouse-On-Mouse) Elite(R) IgG HRP Immunodetection Kit

Immunohistochemistry: M.O.M.(R) (Mouse-On-Mouse) Elite(R) IgG HRP Immunodetection Kit [PK-2200-NB]

Immunohistochemistry: M.O.M.(R) (Mouse-On-Mouse) Elite(R) IgG HRP Immunodetection Kit [PK-2200-NB]

Immunohistochemistry: M.O.M.(R) (Mouse-On-Mouse) Elite(R) IgG HRP Immunodetection Kit [PK-2200-NB] - Mouse, Newborn (eye, double label): GFAP (m), M.O.M.(R) (Mouse-On-Mouse) Elite(R) IgG HRP Immunodetection kit, Vector NovaRED substrate (red); Synapsin (m), MOM Peroxidase Kit, DAB+Ni substrate (gray/black). Note contrast with endogenous pigment (brown) seen in surrounding tissues.
Immunohistochemistry: M.O.M.(R) (Mouse-On-Mouse) Elite(R) IgG HRP Immunodetection Kit [PK-2200-NB]

Immunohistochemistry: M.O.M.(R) (Mouse-On-Mouse) Elite(R) IgG HRP Immunodetection Kit [PK-2200-NB]

Immunohistochemistry: M.O.M.(R) (Mouse-On-Mouse) Elite(R) IgG HRP Immunodetection Kit [PK-2200-NB] - Mouse, Newborn (tongue, double label): Synapsin (m),, Vector NovaRED substrate (red); Desmin (m), M.O.M.(R) (Mouse-On-Mouse) Elite(R) IgG HRP Immunodetection kit, DAB+Ni substrate (gray/black).
M.O.M.(R) (Mouse-On-Mouse) Elite(R) IgG HRP Immunodetection Kit [PK-2200-NB] - M.O.M.(R) (Mouse-On-Mouse) Elite(R) IgG HRP Immunodetection kit

Kit Contents for M.O.M.(R) (Mouse-On-Mouse) Elite(R) IgG HRP Immunodetection Kit

  • M.O.M.(R) (Mouse-On-Mouse) Biotinylated Anti-Mouse IgG Reagent (0.1 mL)
  • M.O.M.(R) (Mouse-On-Mouse) Ig Blocking Reagent (1 mL)
  • M.O.M.(R) (Mouse-On-Mouse) Protein Concentrate (6 mL)
  • VECTASTAIN(R) Elite ABC Reagent A (1 mL)
  • VECTASTAIN(R) Elite ABC Reagent B (1 mL)

Formulation, Preparation, and Storage

Concentration

Concentration is not relevant for this product. Please see the protocols for proper use of this product.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at 4C. Do not freeze.

Background: M.O.M.(R) (Mouse-On-Mouse) Elite(R) IgG

The Vector(R) M.O.M.(R) (Mouse-On-Mouse) Immunodetection Kits are specifically designed to reduce endogenous mouse IgG staining when using mouse primary antibodies on mouse tissue. The inability of an anti-mouse secondary antibody to distinguish between a primary antibody produced in mice and the endogenous mouse immunoglobulins present in mouse tissue results in high background staining which obscures specific staining. This problem can be essentially eliminated by using a Vector(R) M.O.M.(R) (Mouse-On-Mouse) Elite(R) IgG HRP Immunodetection Kit. The result is clear, crisp specific staining of the antigens of interest. The Vector(R) M.O.M.(R) (Mouse-On-Mouse) Elite(R) IgG HRP Immunodetection Kit contains a proprietary Mouse IgG Blocking Reagent and is based on the simple and easy to follow VECTASTAIN ABC reagent protocols; no tedious calculations or antibody pre-binding steps are required. Excellent staining results for a once difficult application have now become routine with the Vector(R) M.O.M.(R) System.

Additional M.O.M.(R) (Mouse-On-Mouse) Elite(R) IgG Products

Product Documents for M.O.M.(R) (Mouse-On-Mouse) Elite(R) IgG HRP Immunodetection Kit

Certificate of Analysis

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Product Specific Notices for M.O.M.(R) (Mouse-On-Mouse) Elite(R) IgG HRP Immunodetection Kit

This product is manufactured by Vector Laboratories and distributed by Novus Biologicals.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

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