Mouse Angiopoietin-like Protein 7/ANGPTL7 Antibody (538401)

R&D Systems | Catalog # MAB4960

R&D Systems

Key Product Details

Species Reactivity

Mouse

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2B Clone # 538401
View all Angiopoietin-like Protein 7/ANGPTL7 Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant mouse Angiopoietin-like Protein 7/ANGPTL7
Gln22-Pro337 (predicted)
Accession # Q8R1Q3

Specificity

Detects mouse Angiopoietin-like Protein 7/ANGPTL7 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) Angiopoietin-like 1, 5, 7, recombinant mouse (rm) Angiopoietin-like 2, 3, 4, 6, rhAngiopoietin-1, rhAngiopoietin-4, rmAngiopoietin-2, or rmAngiopoietin-3 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for Mouse Angiopoietin-like Protein 7/ANGPTL7 Antibody (538401)

Detection of Mouse Angiopoietin-like Protein 7/ANGPTL7 antibody by Western Blot.

Detection of Mouse Angiopoietin-like Protein 7/ANGPTL7 by Western Blot.

Western blot shows lysates of mouse uterus tissue. PVDF membrane was probed with 1 µg/mL of Rat Anti-Mouse Angiopoietin-like Protein 7/ANGPTL7 Monoclonal Antibody (Catalog # MAB4960) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Angiopoietin-like Protein 7/ANGPTL7 at approximately 53 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Angiopoietin-like Protein 7/ANGPTL7 antibody in Mouse Brain by Immunohistochemistry (IHC-Fr).

Angiopoietin-like Protein 7/ANGPTL7 in Mouse Brain.

Angiopoietin-like Protein 7/ANGPTL7 was detected in perfusion fixed frozen sections of mouse brain using Rat Anti-Mouse Angiopoietin-like Protein 7/ANGPTL7 Monoclonal Antibody (Catalog # MAB4960) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in glia. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Angiopoietin-like Protein 7/ANGPTL7 antibody in Mouse Brain by Immunohistochemistry (IHC-Fr).

Angiopoietin-like Protein 7/ANGPTL7 in Mouse Brain.

Angiopoietin-like Protein 7/ANGPTL7 was detected in perfusion fixed frozen sections of mouse brain using Rat Anti-Mouse Angiopoietin-like Protein 7/ANGPTL7 Monoclonal Antibody (Catalog # MAB4960) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neurons. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Applications for Mouse Angiopoietin-like Protein 7/ANGPTL7 Antibody (538401)

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Perfusion fixed frozen sections of mouse brain

Western Blot

1 µg/mL
Sample: Mouse uterus tissue

Reviewed Applications

Read 1 review rated 5 using MAB4960 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Angiopoietin-like Protein 7/ANGPTL7

Angiopoietin-like Protein 7 (ANGPTL7), also known as Corneal-Derived Transcript 6 (CDT6), is a secreted, 45‑50 kDa glycoprotein member of the angiopoietin‑like family of molecules. Members of this protein family contain an N-terminal coiled coil domain and a C-terminal fibrinogen-like domain (1‑3). Mature mouse ANGPTL7 is 316 amino acids (aa) in length. It shares 88% and 98% aa sequence identity with human and rat ANGPTL7, respectively. The ANGPTL7 monomer forms homotetramers via its coiled coil domain (4, 5). ANGPTL7 is expressed in the corneal stroma, trabecular meshwork, and sclera (3, 5). Its production is up‑regulated in trabecular meshwork cells by glucocorticoids and TGF-beta and in cartilage by TNF-alpha (5-7). ANGPTL7 expression is up‑regulated under conditions of increased ocular pressure (IOP). Since IOP is a risk factor for glaucoma, ANGPTL7 may represent a defense mechanism to reduce pressure on the retina. It appears that IOP is a consequence of increased ECM deposition, a major component of which is fibronectin. In this regard, ANGPTL7 is reported to both decrease fibronectin expression and promote MMP1 expression, actions that would tend to reduce organized ECM deposition (8). When overexpressed in tumor cells, it promotes collagen and proteoglycan deposition but inhibits tumor xenograft progression and tumor angiogenesis (4). ANGPTL7, when used in combination with SCF, Thrombopoietin, IGF-II, and FGF acidic, enhances the expansion and engraftment of human and mouse hematopoietic stem cells (9).

References

  1. Oike, Y. et al. (2009) Circ. J. 73:2192.
  2. Miida, T. and S. Hirayama (2010) Curr. Opin. Lipidol. 21:70.
  3. Peek, R. et al. (1998) Invest. Opthalmol. Vis. Sci. 39:1782.
  4. Peek, R. et al. (2002) J. Biol. Chem. 277:686.
  5. Kuchtey, J. et al. (2008) Invest. Opthalmol. Vis. Sci. 49:3438.
  6. Nehme, A. et al. (2009) BMC Med. Genomics 2:58.
  7. Stevens, A.L. et al. (2008) Arthritis Rheum. 58:489.
  8. Comes, N. et al. (2011) Genes Cells 16:243.
  9. Zhang, C.C. et al. (2006) Nat. Med. 12:240.

Long Name

Angiopoietin-like Protein 7

Alternate Names

ANGPTL7, AngX, CDT6

Entrez Gene IDs

10218 (Human); 654812 (Mouse); 102127960 (Cynomolgus Monkey); 714204 (Rhesus Macaque)

Gene Symbol

ANGPTL7

UniProt

Q8R1Q3

Additional Angiopoietin-like Protein 7/ANGPTL7 Products

Product Documents for Mouse Angiopoietin-like Protein 7/ANGPTL7 Antibody (538401)

Certificate of Analysis

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Product Specific Notices for Mouse Angiopoietin-like Protein 7/ANGPTL7 Antibody (538401)

For research use only

Customer Reviews for Mouse Angiopoietin-like Protein 7/ANGPTL7 Antibody (538401) (1)

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  • Mouse Angiopoietin-like Protein 7/ANGPTL7 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Kidney tissue
    Species: Mouse
    Verified Customer | Posted 11/18/2021
    Mouse Angiopoietin-like Protein 7/ANGPTL7 Antibody (538401) MAB4960

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