Mouse beta -Actin Antibody

beta -Actin Antibody in Mouse Stomach viaseqIF™ staining on COMET™​ beta -Actin was detected in perfusion fixed paraffin-embedded sections of mouse Stomach using Rat Anti-Mouse beta -Actin, Monoclonal Antibody (Catalog #MAB11702) at 0.25ug/mL at 37° Celsius for 2 minutes.Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog #TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 555 Goat anti-Rat IgG Secondary Antibody at 1:100 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR555RT) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cell membrane. Protocol available in COMET™ Panel Builder.​

beta -Actin Antibody in Mouse colon via seqIF™ staining on COMET™ beta -Actin was detected in immersion fixed paraffin-embedded sections of mouse colon using Rat Anti-Mouse beta -Actin, Monoclonal Antibody (Catalog # MAB11702) at 0.25ug/mL at 37° Celsius for 2 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 555 Goat anti-Rat IgG Secondary Antibody at 1:100 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR555RT) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cytoplasm. Protocol available in COMET™ Panel Builder.​​

beta -Actin Antibody in Mouse liver via seqIF™ staining on COMET™ beta -Actin was detected in immersion fixed paraffin-embedded sections of mouse liver using Rat Anti-Mouse beta -Actin, Monoclonal Antibody (Catalog # MAB11702) at 0.25ug/mL at 37° Celsius for 2 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 555 Goat anti-Rat IgG Secondary Antibody at 1:100 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR555RT) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cytoplasm. Protocol available in COMET™ Panel Builder.

Detection of beta ‑Actin in Mouse Heart. beta ‑Actin was detected in perfusion fixed paraffin-embedded sections of mouse heart using Rat Anti-Mouse beta ‑Actin Monoclonal Antibody (Catalog # MAB11702) at 0.05 µg/ml overnight at 4 °C. Tissue was stained using the HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of beta ‑Actin in Mouse Liver. beta ‑Actin was detected in perfusion fixed paraffin-embedded sections of mouse liver using Rat Anti-Mouse beta ‑Actin Monoclonal Antibody (Catalog # MAB11702) at 0.05 µg/ml overnight at 4 °C. Tissue was stained using the HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of beta ‑Actin in Mouse Lung. beta ‑Actin was detected in perfusion fixed paraffin-embedded sections of mouse lung using Rat Anti-Mouse beta ‑Actin Monoclonal Antibody (Catalog # MAB11702) at 0.05 µg/ml overnight at 4 °C. Tissue was stained using the HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of beta ‑Actin in 3T3‑L1 Mouse Cell Line. beta ‑Actin was detected in immersion fixed 3T3‑L1 mouse embryonic fibroblast adipose-like cell line using Rat Anti-Mouse beta ‑Actin Monoclonal Antibody (Catalog # MAB11702) at 8 µg/ml for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to the cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of Human, Mouse and Rat beta ‑Actin by Western Blot. Western Blot shows lysates of human cerebellum, mouse cerebellum, rat cerebellum, C2C12 mouse myoblast cell line and NIH‑3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 0.5 µg/ml of Rat Anti-Mouse beta ‑Actin Monoclonal Antibody (Catalog # MAB11702) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for beta ‑Actin at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Detection of Mouse beta ‑Actin by Simple Western^TM. Left: Simple Western lane view shows lysates of C2C12 mouse myoblast cell line, loaded at 0.1 mg/ml. A specific band was detected for beta ‑Actin at approximately 49 kDa (as indicated) using both 10 µg/ml and 50 µg/ml of Rat Anti-Mouse beta ‑Actin Monoclonal Antibody (Catalog # MAB11702) followed by 1:50 dilution of HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005) in Milk-free Antibody Diluent (Catalog # 043-524). This experiment was conducted under reducing conditions and using the 12-230kDa separation system. Right: Simple Western electropherogram showing the same Rat Anti-Mouse beta ‑Actin Monoclonal Antibody (Catalog # MAB11702) tested at 10 µg/ml (blue line) and 50 µg/ml (green line) in the C2C12 mouse myoblast cell line.