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Mouse BOC Antibody

Catalog #: AF2385
6 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF2385
AF2385-SP

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Detection of BOC in Mouse Embryo.
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Citations (6)
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Mouse BOC Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse BOC in direct ELISAs and Western blots. In Western blots, approximately 25% cross‑reactivity with recombinant human BOC is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse BOC
Asp26-Lys508 (Gln ins) Val509-Asp846
Accession # AAH78631
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse BOC
Immunohistochemistry
5-15 µg/mL
Perfusion fixed frozen sections of mouse embryo (E13.5-15.5)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry View Larger

Detection of BOC in Mouse Embryo. BOC was detected in perfusion fixed frozen sections of mouse embryo using Goat Anti-Mouse BOC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2385) at 15 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004) or the HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the membrane of the neural tube, spinal cord. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BOC

BOC and CDO (cell adhesion molecule-related/down-regulated by oncogenes) are cell surface receptors belonging to the Ig/fibronectin type III (FNIII) repeat family. Both proteins have five Ig repeats and three FNIII repeats in their extracellular domains, an arrangement shared by Robo proteins. BOC and CDO are co-expressed in muscle precursor cells during development. BOC associates with CDO in cis and forms complexes with cadherins to regulate myogenic differentiation.

Long Name
Brother of CDO
Entrez Gene IDs
91653 (Human); 117606 (Mouse)
Alternate Names
Boc homolog (mouse); BOC; brother of CDO; Protein BOC

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Citations for Mouse BOC Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Novel Roles of Sonic Hedgehog Signaling in Retinal Patterning and Neurogenesis During Mammalian Eye Development
    Authors: Krueger, MR;Cheema, SK;Simo, S;Levine, EM;Brown, NL;La Torre, A;
    bioRxiv : the preprint server for biology
    Species: Mouse, Transgenic Mouse
    Sample Types: Whole Tissue
    Applications: Immunohistochemistry
  2. Coordination of canonical and noncanonical Hedgehog signalling pathways mediated by WDR11 during primordial germ cell development
    Authors: Lee, J;Kim, Y;Ataliotis, P;Kim, HG;Kim, DW;Bennett, DC;Brown, NA;Layman, LC;Kim, SH;
    Scientific reports
    Species: Mouse
    Sample Types: Cell Lysate
    Applications: Western Blot
  3. Ptch2/Gas1 and Ptch1/Boc differentially regulate Hedgehog signalling in murine primordial germ cell migration
    Authors: Y Kim, J Lee, M Seppala, MT Cobourne, SH Kim
    Nat Commun, 2020-04-24;11(1):1994.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. SGTb regulates a surface localization of a guidance receptor BOC to promote neurite outgrowth
    Authors: TA Vuong, SJ Lee, YE Leem, JR Lee, GU Bae, JS Kang
    Cell. Signal., 2019-01-09;55(0):100-108.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  5. Distinct structural requirements for CDON and BOC in the promotion of Hedgehog signaling
    Authors: Jane Y. Song, Alexander M. Holtz, Justine M. Pinskey, Benjamin L. Allen
    Developmental Biology
  6. Segregation of ipsilateral retinal ganglion cell axons at the optic chiasm requires the Shh receptor Boc.
    Authors: Fabre PJ, Shimogori T, Charron F
    J. Neurosci., 2010-01-06;30(0):266.
    Species: Mouse, Rat
    Sample Types: Whole Tissue
    Applications: IHC-P

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