Mouse Cadherin-17 Antibody (1262C)
R&D Systems | Catalog # MAB8524
Recombinant Monoclonal Antibody.
Key Product Details
Species Reactivity
Mouse
Applications
Immunohistochemistry, Western Blot, Simple Western
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 1262C
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Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Cadherin-17
Glu22-Met786
Accession # Q9R100
Glu22-Met786
Accession # Q9R100
Specificity
Detects mouse Cadherin-17 in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Mouse Cadherin-17 Antibody (1262C)
Detection of Mouse Cadherin‑17 by Western Blot.
Western blot shows lysates of mouse colon tissue and mouse spleen tissue. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Mouse Cadherin-17 Monoclonal Antibody (Catalog # MAB8524) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Cadherin-17 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Cadherin‑17 in Mouse Intestine.
Cadherin-17 was detected in perfusion fixed frozen sections of mouse intestine using Rabbit Anti-Mouse Cadherin-17 Monoclonal Antibody (Catalog # MAB8524) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to plasma membranes. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Detection of Mouse Cadherin‑17 by Simple WesternTM.
Simple Western lane view shows lysates of mouse colon tissue, loaded at 0.2 mg/mL. A specific band was detected for Cadherin‑17 at approximately 153 kDa (as indicated) using 1 µg/mL of Rabbit Anti-Mouse Cadherin‑17 Monoclonal Antibody (Catalog # MAB8524). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Mouse Cadherin-17 Antibody (1262C)
Application
Recommended Usage
Immunohistochemistry
1-25 µg/mL
Sample: Perfusion fixed frozen sections of mouse intestine
Sample: Perfusion fixed frozen sections of mouse intestine
Simple Western
1 µg/mL
Sample: Mouse colon tissue
Sample: Mouse colon tissue
Western Blot
0.1 µg/mL
Sample: Mouse colon tissue and mouse spleen tissue
Sample: Mouse colon tissue and mouse spleen tissue
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Cadherin-17
Alternate Names
Cadherin17, CDH17
Gene Symbol
CDH17
UniProt
Additional Cadherin-17 Products
Product Documents for Mouse Cadherin-17 Antibody (1262C)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Mouse Cadherin-17 Antibody (1262C)
For research use only
Related Research Areas
Citations for Mouse Cadherin-17 Antibody (1262C)
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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