Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Human, Mouse, Rat, Transgenic Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, Simple Western, Immunoprecipitation

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunocytochemistry, ISH-IHC

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Carbonic Anhydrase IV/CA4 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Carbonic Anhydrase IV/CA4
Glu18-Ser277
Accession # Q64444

Specificity

Detects mouse Carbonic Anhydrase IV/CA4 in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross‑reactivity with recombinant human CA4 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse Carbonic Anhydrase IV/CA4 Antibody

Detection of Mouse Carbonic Anhydrase IV/CA4 antibody by Western Blot.

Detection of Mouse Carbonic Anhydrase IV/CA4 by Western Blot.

Western blot shows lysates of mouse kidney tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse Carbonic Anhydrase IV/CA4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2414) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Carbonic Anhydrase IV/CA4 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Carbonic Anhydrase IV/CA4 antibody in Mouse Kidney by Immunohistochemistry (IHC-Fr).

Carbonic Anhydrase IV/CA4 in Mouse Kidney.

Carbonic Anhydrase IV/CA4 was detected in perfusion fixed frozen sections of mouse kidney using Goat Anti-Mouse Carbonic Anhydrase IV/CA4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2414) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in epithelial cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Detection of Mouse Carbonic Anhydrase IV/CA4 antibody by Simple WesternTM.

Detection of Mouse Carbonic Anhydrase IV/CA4 by Simple WesternTM.

Simple Western lane view shows lysates of mouse lung tissue and mouse kidney tissue, loaded at 0.2 mg/mL. A specific band was detected for Carbonic Anhydrase IV/CA4 at approximately 51-52 kDa (as indicated) using 12.5 µg/mL of Goat Anti-Mouse Carbonic Anhydrase IV/CA4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2414) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Detection of Carbonic Anhydrase IV/CA4 by Western Blot

Detection of Carbonic Anhydrase IV/CA4 by Western Blot

Basal pH level and ability to capacitate of CAII−/− CAIV−/− sperm is not altered.A, immunoblots were performed with protein extracts from untreated or capacitated sperm from WT (+/+) and CAII−/− CAIV−/− (−/−) mice. CAII−/−CAIV−/− sperm do not show any changes in the protein phosphorylation pattern induced by capacitation (Cap) compared with WT sperm. B, CAII−/− CAIV−/− sperm do not show any defects in intracellular alkalization upon stimulation with ammonium chloride. Sperm of both WT (black trace) and CAII−/− CAIV−/− (gray trace) mice were perfused continuously with HS buffer and 10 mm ammonium chloride as indicated. Shown are averaged normalized traces of 34 WT and 39 CAII−/− CAIV−/− sperm of two animals, respectively. A.U., arbitrary units. C, CAII−/− sperm show a reduced CAIV protein level, whereas the amount of CAII protein in sperm from CAIV−/− mice corresponds to that of WT mice. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/26487715), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse Carbonic Anhydrase IV/CA4 Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample:  Perfusion fixed frozen sections of mouse kidney

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Mouse Carbonic Anhydrase IV (Catalog # 2414-CA), see our available Western blot detection antibodies

Simple Western

12.5 µg/mL
Sample: Mouse lung tissue and mouse kidney tissue

Western Blot

0.25 µg/mL
Sample: Mouse kidney tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Carbonic Anhydrase IV/CA4

Carbonic anhydrase (CA) catalyzes the reversible reaction of CO2 + H2O = HCO3- + H+, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1). Topics in a CA meeting (6th International Conference on the CAs, June 20‑25, 2003, Slovakia) ranged from use of CAs as markers for tumor and hypoxia in clinic, as nutritional supplement in milk, and as a tool for CO2 removal and mosquito control in industry. The deduced amino acid sequence of mouse CA4 consists of a signal peptide (residues 1‑17), an ectodomain (residues 18‑277) and a pro peptide (residues 278‑305), which is removed in the mature form (2). The native enzyme is attached to the membrane by a GPI‑anchor. Recombinant mouse CA4 corresponds to the ectodomain and exhibits the activity as described in Activity Assay Protocol.

References

  1. Hewett-Emmett, D. and R.E. Tashian (1996) Mol. Phylogenet. Evol. 5:50.
  2. Tamai, S. et al. (1996) Biochem. Genet. 34:31.

Alternate Names

CA4, RP17

Entrez Gene IDs

762 (Human); 12351 (Mouse)

Gene Symbol

CA4

UniProt

Q64444

Additional Carbonic Anhydrase IV/CA4 Products

Product Documents for Mouse Carbonic Anhydrase IV/CA4 Antibody

Certificate of Analysis

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Product Specific Notices for Mouse Carbonic Anhydrase IV/CA4 Antibody

For research use only

Citations for Mouse Carbonic Anhydrase IV/CA4 Antibody

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Protocols

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