Mouse CCR2 Antibody (475301)

R&D Systems | Catalog # MAB55381

R&D Systems
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Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Flow Cytometry

Cited:

Neutralization, Flow Cytometry, CyTof, FACS, In vivo assay

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2B Clone # 475301
View all CCR2 Primary Antibodies »

Product Specifications

Immunogen

L1.2 mouse pro-B cell line transfected with mouse CCR2

Specificity

Detects mouse CCR2 transfectants but not the parental cell line in flow cytometry. Does not detect HEK293 human embryonic kidney cell line transfected with mouse CCR5 in flow cytometry.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for Mouse CCR2 Antibody (475301)

Detection of CCR2 antibody in NS0 Mouse Cell Line Transfected with Mouse CCR2 and eGFP antibody by Flow Cytometry.

Detection of CCR2 in NS0 Mouse Cell Line Transfected with Mouse CCR2 and eGFP by Flow Cytometry.

NS0 mouse myeloma cell line transfected with either (A) mouse CCR2 or (B) irrelevant transfectants and eGFP was stained with Rat Anti-Mouse/Rat CCR2 Monoclonal Antibody (Catalog # MAB55381) followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B). Quadrant markers were set based on isotype control (Rat IgG2B,Catalog # MAB0061; data not shown). View our protocol for Staining Membrane-associated Proteins.
Detection of CCR2 by Flow Cytometry

Detection of CCR2 by Flow Cytometry

Inflammatory monocytes are indispensable for cortical demyelination in Th/+ mice. a Representative density plots of inflammatory monocytes (CD45+CD11b+Ly6ChiLy6G-) isolated from the cortex of Th/+ CCR2+/+ and Th/+ CCR2−/− mice on day 2 post stereotactic injection. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/28386765), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse CCR2 Antibody (475301)

Application
Recommended Usage

Flow Cytometry

0.25 µg/106 cells
Sample: NS0 Mouse Cell Line Transfected with Mouse CCR2 and eGFP

Reviewed Applications

Read 1 review rated 4 using MAB55381 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CCR2

CCR2, also known as CD192, is a 38 kDa 7TM chemokine receptor that preferentially binds CCL2, CCL7, and CCL13. CCR2 is expressed by multiple hematopoietic cells, endothelial cells, fibroblasts, neurons, and smooth muscle cells. It functions as an HIV fusion co-factor and facilitates T cell recruitment during inflammation. Two alternate splice forms (CCR2A and CCR2B) differ only by the addition of 14 amino acids to the intracellular carboxyl terminal. Mouse CCR2 shares 67% and 95% aa sequence identity with human and rat CCR2, respectively.

Alternate Names

CC-CKR-2, CCR2, CCR2B, CD192, CKR2, CKR2A, CMKBR2, FLJ78302, MCP-1-R

Entrez Gene IDs

729230 (Human); 12772 (Mouse); 60463 (Rat)

Gene Symbol

CCR2

Additional CCR2 Products

Product Documents for Mouse CCR2 Antibody (475301)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse CCR2 Antibody (475301)

For research use only

Citations for Mouse CCR2 Antibody (475301)

Customer Reviews for Mouse CCR2 Antibody (475301) (1)

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  • Mouse CCR2 Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Lymph node cells
    Species: Mouse
    Verified Customer | Posted 04/04/2019
    Lymph node cells from EAE mice stained for viable cells and CCR2 (blue) and FMO for CCR2 (red).
    Experimental autoimmune encephalomyelitis was induced in C57BL6/J mice, and periaortic and inguinal lymph node cells were isolated when the animals were at the onset of symptoms. Cells were stained for CCR2, CD45, CD11b, CD11c, CD4, CD8, CX3CR1, CCR5, CCR7, and CXCR4, plus for viability to exclude dead cells. Viable cells stained for CCR2 (blue) and FMO sample for CCR2 (stained for all the markers except for CCR2; red).
    Mouse CCR2 Antibody (475301) MAB55381

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