Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Western Blot, Immunocytochemistry

Cited:

Immunohistochemistry, FACS

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2B Clone # 612722
View all CD163 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line, NS0-derived recombinant mouse CD163
Val39-Thr1045
Accession # Q2VLH6

Specificity

Detects mouse CD163 in direct ELISA.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for Mouse CD163 Antibody

Detection of Mouse CD163 by Western Blot.

Western Blot shows lysates of mouse liver. PVDF membrane was probed with 2 µg/ml of Rat Anti-Mouse CD163 Monoclonal Antibody (Catalog # MAB7435) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for CD163 at approximately 140 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Western Blot Buffer Group 1.

Detection of CD163 in Neuro2A cells (Positive).

CD163 was detected in fixed Neuro2A cells (Positive) using Rat Anti-Mouse CD163 Monoclonal Antibody (Catalog # MAB7435) at 8 µg/ml for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to the membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Mouse CD163 Antibody

Application
Recommended Usage

Immunocytochemistry

3-25 µg/mL
Sample: Formalin fixed Neuro2A cells (Positive)

Western Blot

2 µg/mL
Sample: Mouse liver

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD163

CD163, also known as M130 and  p155, is a 130-160 kDa type I transmembrane protein belonging to group B of the cysteine-rich scavenger receptor family (1). Mature mouse CD163 consists of a 1007 amino acid (aa) extracellular domain (ECD) with 9 scavenger receptor cysteine-rich (SRCR) domains, a 21 aa transmembrane segment, and a 55 aa cytoplasmic domain (2). Alternative splicing of mouse CD163 generates an isoform with a substitution in the cytoplasmic region. Within the ECD, mouse CD163 shares 75% and 90% aa sequence identity with human and rat CD163, respectively. CD163 is expressed on monocytes and macrophages and is inducible by immunosuppressant glucocorticoids and IL-10 (3-5). A soluble form is shed from the cell surface by TACE or neutrophil elastase mediated cleavage (6, 7) in response to oxidative stress, Prostaglandin F2a stimulation, or the activation of Fc gamma receptors, TLR1, 2, 5, or 6 (8-10). CD163 mediates monocyte binding to bacteria, leading to the release of inflammatory cytokines (11). It is essential for the circulatory clearance of hemoglobin-haptoglobin (Hb-Hp) complexes as well as free hemoglobin (12, 13). It can also mediate monocyte-erythroblast adhesion and promote erythroblast expansion (14). CD163 binds and internalizes the cytokine TWEAK, and the ratio of soluble CD163 to TWEAK in the plasma is elevated during atherosclerosis (15, 16).

References

  1. Kowal, K. et al. (2011) Folia Histochem. Cytobiol. 49:365.
  2. Law, S.K.A. et al. (1993) Eur. J. Immunol. 23:2320.
  3. Hogger, P. et al. (1998) J. Immunol. 161:1883.
  4. Sulahian, T.H. et al. (2000) Cytokine 12:1312.
  5. Buechler, C. et al. (2000) J. Leukoc. Biol. 67:97.
  6. Etzerodt, A. et al. (2010) J. Leukoc. Biol. 88:1201.
  7. Moreno, J.A. et al. (2012) Eur. Heart J. 33:252.
  8. Weaver, L.K. et al. (2006) J. Leukoc. Biol. 80:26.
  9. Timmermann, M. and P. Hogger (2005) Free Radic. Biol. Med. 39:98.
  10. Sulahian, T.H. et al. (2004) J. Leukoc. Biol. 76:271.
  11. Fabriek, B.O. et al. (2009) Blood 113:887.
  12. Kristiansen, M. et al. (2001) Nature 409:198.
  13. Schaer, D.J. et al. (2006) Blood 107:373.
  14. Fabriek, B.O. et al. (2007) Blood 109:5223.
  15. Bover, L.C. et al. (2007) J. Immunol. 178:8183.
  16. Moreno, J.A. et al. (2009) Atherosclerosis 207:103.

Alternate Names

CD163, GHI/61, HbSR, M130, RM3/1

Entrez Gene IDs

9332 (Human); 93671 (Mouse); 312701 (Rat)

Gene Symbol

CD163

UniProt

Q2VLH6

Additional CD163 Products

Product Documents for Mouse CD163 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse CD163 Antibody

For research use only

Citations for Mouse CD163 Antibody

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Protocols

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