Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse, Transgenic Mouse

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all CLEC4F/CLECSF13 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse CLEC4F/CLECSF13
Ala65-Gly548
Accession # P70194

Specificity

Detects mouse CLEC4F/CLECSF13 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse CLEC4F/CLECSF13 Antibody

CLEC4F/CLECSF13 antibody in Mouse Liver by Immunohistochemistry (IHC-Fr).

CLEC4F/CLECSF13 in Mouse Liver.

CLEC4F/CLECSF13 was detected in perfusion fixed frozen sections of mouse liver using Goat Anti-Mouse CLEC4F/CLECSF13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2784) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to Kupffer cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Detection of Mouse CLEC4F/CLECSF13 by Western Blot

Detection of Mouse CLEC4F/CLECSF13 by Western Blot

L-glutamine treatment does not ameliorate ischemic injury in SCD mouse liver. (A) Quantitative liver intravital (qLIM) imaging of three different fields of view of SCD mice at baseline and post-L-glutamine administration injected with TXR-dextran. Dotted circle shows loss of blood flow in SCD liver which was comparable in L-glutamine-treated SCD mouse liver. (B) Quantification of the total area (%) of liver with loss of blood flow in SCD mice at baseline and post-L-glutamine treatment. (C) Representative IF images show enhanced CLEC4F and F4/80 expression in SCD liver which was not seen after L-glutamine administration. (D) Western Blot for CLEC4F and CD45 antibodies exhibits increased expression in the liver of SCD mice compared to L-glutamine-treated SCD mouse liver. (E) qRT-PCR analysis exhibits reduced mRNA expression of markers of inflammatory cells (including F4/80, Cd45, CLEC4F, and IL1 beta ) in L-glutamine-treated SCD liver compared to SCD liver at baseline. * denotes p < 0.05, NS, Not significant. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37760853), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse CLEC4F/CLECSF13 Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Perfusion fixed frozen sections of mouse liver

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse CLEC4F/CLECSF13 (Catalog # 2784-CL)

Reviewed Applications

Read 2 reviews rated 5 using AF2784 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CLEC4F/CLECSF13

CLEC4F (C-type lectin domain; family 4, member F; also known as the Kupffer cell receptor and fucose receptor) is an 80 kDa, type II transmembrane glycoprotein member of the C-type lectin superfamily (1‑3). Mature mouse CLEC4F consists of a 42 amino acid (aa) cytoplasmic domain, a 27 aa transmembrane segment, and a 479 aa extracellular domain (ECD) that contains an extended stalk region plus one carbohydrate recognition domain (4, 5). Within the ECD, mouse CLEC4F shares 48% and 79% aa sequence identity with human and rat CLEC4F, respectively. The stalk region of CLEC4F is a coiled coil domain that mediates homotrimer formation (6, 7). CLEC4F is expressed on Kupffer cells in the liver, but not on macrophages in other tissues (8). CLEC4F preferentially binds galactose and N‑acetylgalactosamine in a calcium-dependent manner (6, 9, 10). Its activity at neutral, but not at acidic pH, suggests a capacity to internalize and release ligands into the endosomal system (11).

References

  1. Zelensky, A.N. and J.E. Gready (2005) FEBS J. 272:6179.
  2. Bilzer, M. et al. (2006) Liver Int. 26:1175.
  3. Kuiper, J. et al. (1994) Biochem. J. 299:285.
  4. Accession # P70194.
  5. Hoyle, G.W. and R.L. Hill (1988) J. Biol. Chem. 263:7487.
  6. Fadden, A.J. et al. (2003) Glycobiology 13:529.
  7. Beavil, A.J. et al. (1992) Proc. Natl. Acad. Sci. 89:753.
  8. Haltiwanger, R.S. et al. (1986) J. Biol. Chem. 261:7433.
  9. Coombs, P.J. et al. (2006) Glycobiology 16:1C.
  10. Biessen, E.A.L. et al. (1994) Biochem. J. 299:291.
  11. Lehrman, M.A. et al. (1986) J. Biol. Chem. 261:7426.

Long Name

C-type Lectin Domain Family 4, Member F

Alternate Names

CLECSF13, Galactose Particle Receptor, KCLR, KCR

Entrez Gene IDs

165530 (Human); 51811 (Mouse); 114598 (Rat)

Gene Symbol

CLEC4F

UniProt

P70194

Additional CLEC4F/CLECSF13 Products

Product Documents for Mouse CLEC4F/CLECSF13 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse CLEC4F/CLECSF13 Antibody

For research use only

Related Research Areas

C-type Lectin Family

Citations for Mouse CLEC4F/CLECSF13 Antibody

Customer Reviews for Mouse CLEC4F/CLECSF13 Antibody (2)

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  • Mouse CLEC4F/CLECSF13 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Liver tissue
    Species: Mouse
    Verified Customer | Posted 12/19/2020
    green color: clec4f
    Mouse CLEC4F/CLECSF13 Antibody AF2784
  • Mouse CLEC4F/CLECSF13 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Liver tissue
    Species: Mouse
    Verified Customer | Posted 09/28/2020
    The antibody works very well for IF staining in mouse liver either paraffin and frozen sections.
    Mouse CLEC4F/CLECSF13 Antibody AF2784

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