Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, Simple Western

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all Collectrin Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Collectrin
Glu15-Ile144
Accession # Q9ESG4

Specificity

Detects mouse Collectrin in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant human Collectrin is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Mouse Collectrin Antibody

Detection of Mouse Collectrin antibody by Western Blot.

Detection of Mouse Collectrin by Western Blot.

Western blot shows lysates of mouse kidney tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse Collectrin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4965) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Collectrin at approximately 43 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Collectrin antibody in Mouse Kidney by Immunohistochemistry (IHC-Fr).

Collectrin in Mouse Kidney.

Collectrin was detected in perfusion fixed frozen sections of mouse kidney using Sheep Anti-Mouse Collectrin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4965) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to convoluted tubules. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Detection of Mouse Collectrin antibody by Simple WesternTM.

Detection of Mouse Collectrin by Simple WesternTM.

Simple Western lane view shows lysates of mouse kidney tissue, loaded at 0.2 mg/mL. A specific band was detected for Collectrin at approximately 69 kDa (as indicated) using 10 µg/mL of Sheep Anti-Mouse Collectrin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4965) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Mouse Collectrin Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Perfusion fixed frozen sections of mouse kidney

Simple Western

10 µg/mL
Sample: Mouse kidney tissue

Western Blot

1 µg/mL
Sample: Mouse kidney tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Collectrin

Collectrin (also Transmembrane protein 27) is a 37‑43 kDa member of the MEM27 family of proteins. It is expressed on pancreatic beta -cells where it regulates insulin secretion, and on renal tubular epithelium where it mediates amino acid transport. Mature mouse Collectrin is a 208 amino acid (aa) type I transmembrane glycoptotein. It contains a 127 aa extracellular region (aa 15‑141) and a 60 aa cytoplasmic domain (aa 163‑222). Collectrin dimerizes on the cell surface, and undergoes proteolytic cleavage to generate a 26 kDa soluble fragment. Over aa 15‑144, mouse Collectrin shares 92% and 86% aa identity with rat and human Collectrin, respectively.

Alternate Names

NX17, TMEM27

Entrez Gene IDs

57393 (Human); 57394 (Mouse); 57395 (Rat)

Gene Symbol

CLTRN

UniProt

Q9ESG4

Additional Collectrin Products

Product Documents for Mouse Collectrin Antibody

Certificate of Analysis

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Product Specific Notices for Mouse Collectrin Antibody

For research use only

Citations for Mouse Collectrin Antibody

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Protocols

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