Key Product Details

Species Reactivity

Mouse

Applications

Western Blot

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all Ephrin-A4 Primary Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse Ephrin-A4
Met1-Gly176
Accession # O08542

Specificity

Detects mouse Ephrin-A4 in Western blots. In Western blots, approximately 15% cross‑reactivity with recombinant human (rh) Ephrin-A4 is observed and less than 5% cross-reactivity with rhEphrin-A3, rhEphrin-A5, rhEphrin-B3, recombinant mouse (rm) Ephrin-B2, rmEphrin-A1, and rmEphrin-A2 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Mouse Ephrin-A4 Antibody [Biotin]

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse Ephrin-A4 Fc Chimera (Catalog # 569-A4)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Ephrin-A4

Ephrin-A4, also known as LERK-4 and EFL-4 (1), is a member of the ephrin ligand family which binds members of the Eph receptor family. All ligands share a conserved extracellular sequence, which most likely corresponds to the receptor binding domain. This conserved sequence consists of approximately 125 amino acids and includes four invariant cysteines. The A-class ligands have a GPI anchor following the conserved sequence. Ephrin-A4 has been shown to bind EphA2, EphA3, EphA4, EphA5, EphA6, EphA7, and EphB1 (2, 3). The extracellular domains of human and mouse Ephrin-A4 share 80% amino acid identity. Only membrane-bound or Fc-clustered ligands are capable of activating the receptor in vitro. While soluble monomeric ligands bind the receptor, they do not induce receptor autophosphorylation and activation (2). In vivo, the ligands and receptors display reciprocal expression (3). It has been found that nearly all receptors and ligands are expressed in developing and adult neural tissue (3). The Eph/ephrin families also appear to play a role in angiogenesis (3).

References

  1. Eph Nomenclature Committee [letter] (1997) Cell 90:403.
  2. Flanagan, J.G. and P. Vanderhaeghen (1998) Annu. Rev. Neurosci. 21:309.
  3. Pasquale, E.B. (1997) Curr. Opin. Cell Biol. 9:608.

Alternate Names

EFL-4, EFNA4, EphrinA4, LERK-4

Entrez Gene IDs

1945 (Human); 13639 (Mouse)

Gene Symbol

EFNA4

UniProt

O08542

Additional Ephrin-A4 Products

Product Documents for Mouse Ephrin-A4 Antibody [Biotin]

Certificate of Analysis

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Product Specific Notices for Mouse Ephrin-A4 Antibody [Biotin]

For research use only

Related Research Areas

Ephrin Ligands and Eph Receptors

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