Key Product Details

Species Reactivity

Mouse

Applications

Western Blot, ELISA Detection (Matched Antibody Pair)

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all Fc gamma RII/RIII (CD32/CD16) Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Fc gamma RIIB
Thr40-Arg217
Accession # NP_034317

Specificity

Detects mouse Fc gamma RII/RIII (CD32/CD16) in ELISAs and Western blots. In sandwich immunoassays, less than 10% cross-reactivity with recombinant mouse CD16 is observed and less than 0.1% cross-reactivity with recombinant human CD32 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Mouse Fc gamma RII/RIII (CD32/CD16) Biotinylated Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse Fc gamma  RIIB/CD32b (Catalog # 1460-CD)

Mouse Fc gamma RIIA/CD32a Sandwich Immunoassay

ELISA Detection (Matched Antibody Pair)
Recommended Concentration: 0.1-0.4 µg/mL
Use in combination with these reagents:
  • Capture Reagent: Mouse Fc gamma  RIIB/CD32b Antibody (Catalog # MAB14601)
  • Standard: Recombinant Mouse Fc gamma RIIB/CD32b Protein, CF (Catalog # 1460-CD)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Fc gamma RII/RIII (CD32/CD16)

Fc gamma RII, also named CD32, is a low-affinity receptor for the Fc region of complexed IgG. Three genes for human Fc gamma RII (A, B and C) and one for mouse (Fc gamma RIIB), encoding type I transmembrane proteins, have been identified. The extracellular domain of mouse Fc gamma RII shares approximately 95% amino acid sequence identity with that of mouse Fc gamma RIII/CD16.

Long Name

Fc gamma Receptor II/III

Alternate Names

CD32/CD16

UniProt

NP_034317

Additional Fc gamma RII/RIII (CD32/CD16) Products

Product Documents for Mouse Fc gamma RII/RIII (CD32/CD16) Biotinylated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse Fc gamma RII/RIII (CD32/CD16) Biotinylated Antibody

For research use only

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