Mouse FCRL5/FcRH5 Alexa Fluor™ Plus 594‑conjugated Antibody

R&D Systems | Catalog # AF6757AFP594

R&D Systems

Key Product Details

Species Reactivity

Mouse

Applications

Flow Cytometry, CyTOF-ready

Label

Alexa Fluor Plus 594 (Excitation = 590 nm, Emission = 618 nm)

Antibody Source

Polyclonal Sheep IgG
View all FCRL5 Primary Antibodies »

Product Specifications

Specificity

Detects mouse FCRL5/FcRH3 in direct ELISAs. In direct ELISAs, less than 1% cross-reactivity with recombinant human FCRL5 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Applications for Mouse FCRL5/FcRH5 Alexa Fluor™ Plus 594‑conjugated Antibody

Application
Recommended Usage

CyTOF-ready

Optimal dilution of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilution of this antibody should be experimentally determined.

Spectra Viewer

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Flow Cytometry

Formulation, Preparation, and Storage

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: FCRL5

Fc Receptor-Like 5 (FCRL5), also known as FcRH3 (FcRH5 in human), IRTA2, and CD307e, is a 90‑95 kDa member of the FCRL family of proteins whose amino acid (aa) sequence is reminiscent of that for classical Fc receptors. FCLR molecules are type 1 transmembrane proteins that contain from three to nine immunoglobulin-like domains. They are differentially expressed within the B cell lineage, and can either promote or inhibit B cell proliferation and activation (1, 2, 3). Mature mouse FCRL5 consists of a 470 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 79 aa cytoplasmic region. The ECD contains five Ig-like domains, while the cytoplasmic region possesses one ITAM-like motif and one immunotyrosine inhibitory motif (ITIM) (1, 3, 4). There are two major alleles for FCLR5 in mouse. The first was just described, and is found in BALB/c plus NZB mouse strains. The second is found in C57BL/6 mice, and differs by eleven scattered aa in the ECD. This creates one additional N-linked glycosylation site, and increases the SDS‑PAGE MW by 5 kDa (3). Alternate splicing of mouse FCRL5 generates at least one additional isoform that lacks the first Ig-like domain (aa 3‑90 of the mature molecule) (4). Human FCRL5, by contrast, contains up to nine Ig-like domains in a highly variable ECD, and over common regions, mouse and human FCRL5 share 49% aa sequence identity. CRL5 expression is restricted to mature B lineage cells in lymphoid tissues and blood, and is particularly noted to be expressed on T‑independent marginal zone and B1 B cells (3-8). Its ligation inhibits signaling through the B cell antigen receptor (9). Epstein-Barr virus transformation of B cells induces the up‑regulation of surface FCRL5 by a direct effect of its EBNA2 protein on FCRL5 gene transcription (10). FCRL5 on B cells functions as a receptor for the orthopoxvirus MHC class I-like protein OMCP (11). And based on the literature and R&D Systems testing, both mouse and human FCRL5 will bind to purified IgG with high affinity (5). In human, the FCRL5 gene maps to the 1q21 chromosomal locus, a common site of rearrangements in B cell malignancies. Notably, the FCRL5 protein is preferentially expressed in cell lines with 1q21 abnormalities, and is up‑regulated on tumor cells in some types of B cell malignancies (5, 7, 12‑14). In addition, soluble FCRL5 is elevated in the serum of many B cell leukemia patients (13, 15).

References

  1. Davis, R.S. (2007) Annu. Rev. Immunol. 25:525.
  2. Maltais, L.J. et al. (2006) Nat. Immunol. 7:431.
  3. Won, W-J. et al. (2006) J. Immunol. 177:6815.
  4. Davis, R.S. et al. (2004) Int. Immunol. 16:1343.
  5. Hatzivassiliou, G. et al. (2001) Immunity 14:277.
  6. Miller, I. et al. (2002) Blood 99:2662.
  7. Polson, A.G. et al. (2006) Int. Immunol. 18:1363.
  8. Vidal-Laliena, M. et al. (2005) Cell. Immunol. 236:6.
  9. Haga, C.L. et al. (2007) Proc. Natl. Acad. Sci. 104:9770.
  10. Mohan, J. et al. (2006) Blood 107:4433.
  11. Campbell, J.A. et al. (2010) J. Immunol. 185:28.
  12. Ise, T. et al. (2005) Clin. Cancer Res. 11:87.
  13. Ise, T. et al. (2007) Leukemia 21:169.
  14. Kazemi, T. et al. (2009) Cancer Immunol. Immunother. 58:989.
  15. Ise, T. et al. (2006) Clin. Chem. Lab. Med. 44:594.

Long Name

Fc Receptor-like 5

Alternate Names

BXMAS1, CD307e, FcRH5, IFGP5, IRTA2, PRO820

Entrez Gene IDs

83416 (Human); 329693 (Mouse)

Gene Symbol

FCRL5

UniProt

NP_899045

Additional FCRL5 Products

Product Documents for Mouse FCRL5/FcRH5 Alexa Fluor™ Plus 594‑conjugated Antibody

Certificate of Analysis

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Product Specific Notices for Mouse FCRL5/FcRH5 Alexa Fluor™ Plus 594‑conjugated Antibody


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

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