Key Product Details

Species Reactivity

Mouse

Applications

Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all FCRL5 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse FCRL5/FcRH3
Gln27-Ala496 (predicted)
Accession # NP_899045

Specificity

Detects mouse FCRL5/FcRH3 in direct ELISAs. In direct ELISAs, less than 1% cross-reactivity with recombinant human FCRL5 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Mouse FCRL5 Antibody

Detection of FCRL5/FcRH3 antibody in Mouse Splenocytes antibody by Flow Cytometry.

Detection of FCRL5/FcRH3 in Mouse Splenocytes by Flow Cytometry.

Mouse splenocytes were stained with Sheep Anti-Mouse FCRL5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6757) followed by Allophycocyanin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0127) and Rat Anti-Mouse B220/CD45R PE-conjugated Monoclonal Antibody (Catalog # FAB1217P). Quadrant markers were set based on control antibody staining (Catalog # 5-001-A).

Applications for Mouse FCRL5 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: Mouse splenocytes

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
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  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: FCRL5

Fc Receptor-Like 5 (FCRL5), also known as FcRH3 (FcRH5 in human), IRTA2, and CD307e, is a 90‑95 kDa member of the FCRL family of proteins whose amino acid (aa) sequence is reminiscent of that for classical Fc receptors. FCLR molecules are type 1 transmembrane proteins that contain from three to nine immunoglobulin-like domains. They are differentially expressed within the B cell lineage, and can either promote or inhibit B cell proliferation and activation (1, 2, 3). Mature mouse FCRL5 consists of a 470 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 79 aa cytoplasmic region. The ECD contains five Ig-like domains, while the cytoplasmic region possesses one ITAM-like motif and one immunotyrosine inhibitory motif (ITIM) (1, 3, 4). There are two major alleles for FCLR5 in mouse. The first was just described, and is found in BALB/c plus NZB mouse strains. The second is found in C57BL/6 mice, and differs by eleven scattered aa in the ECD. This creates one additional N-linked glycosylation site, and increases the SDS‑PAGE MW by 5 kDa (3). Alternate splicing of mouse FCRL5 generates at least one additional isoform that lacks the first Ig-like domain (aa 3‑90 of the mature molecule) (4). Human FCRL5, by contrast, contains up to nine Ig-like domains in a highly variable ECD, and over common regions, mouse and human FCRL5 share 49% aa sequence identity. CRL5 expression is restricted to mature B lineage cells in lymphoid tissues and blood, and is particularly noted to be expressed on T‑independent marginal zone and B1 B cells (3-8). Its ligation inhibits signaling through the B cell antigen receptor (9). Epstein-Barr virus transformation of B cells induces the up‑regulation of surface FCRL5 by a direct effect of its EBNA2 protein on FCRL5 gene transcription (10). FCRL5 on B cells functions as a receptor for the orthopoxvirus MHC class I-like protein OMCP (11). And based on the literature and R&D Systems testing, both mouse and human FCRL5 will bind to purified IgG with high affinity (5). In human, the FCRL5 gene maps to the 1q21 chromosomal locus, a common site of rearrangements in B cell malignancies. Notably, the FCRL5 protein is preferentially expressed in cell lines with 1q21 abnormalities, and is up‑regulated on tumor cells in some types of B cell malignancies (5, 7, 12‑14). In addition, soluble FCRL5 is elevated in the serum of many B cell leukemia patients (13, 15).

References

  1. Davis, R.S. (2007) Annu. Rev. Immunol. 25:525.
  2. Maltais, L.J. et al. (2006) Nat. Immunol. 7:431.
  3. Won, W-J. et al. (2006) J. Immunol. 177:6815.
  4. Davis, R.S. et al. (2004) Int. Immunol. 16:1343.
  5. Hatzivassiliou, G. et al. (2001) Immunity 14:277.
  6. Miller, I. et al. (2002) Blood 99:2662.
  7. Polson, A.G. et al. (2006) Int. Immunol. 18:1363.
  8. Vidal-Laliena, M. et al. (2005) Cell. Immunol. 236:6.
  9. Haga, C.L. et al. (2007) Proc. Natl. Acad. Sci. 104:9770.
  10. Mohan, J. et al. (2006) Blood 107:4433.
  11. Campbell, J.A. et al. (2010) J. Immunol. 185:28.
  12. Ise, T. et al. (2005) Clin. Cancer Res. 11:87.
  13. Ise, T. et al. (2007) Leukemia 21:169.
  14. Kazemi, T. et al. (2009) Cancer Immunol. Immunother. 58:989.
  15. Ise, T. et al. (2006) Clin. Chem. Lab. Med. 44:594.

Long Name

Fc Receptor-like 5

Alternate Names

BXMAS1, CD307e, FcRH5, IFGP5, IRTA2, PRO820

Entrez Gene IDs

83416 (Human); 329693 (Mouse)

Gene Symbol

FCRL5

UniProt

NP_899045

Additional FCRL5 Products

Product Documents for Mouse FCRL5 Antibody

Certificate of Analysis

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Product Specific Notices for Mouse FCRL5 Antibody

For research use only

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