Mouse GM-CSF Antibody (MP122E9)

R&D Systems | Catalog # MAB415

R&D Systems

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Mouse

Cited:

Human, Mouse, Transgenic Mouse

Applications

Validated:

Western Blot, ELISA Capture (Matched Antibody Pair), Neutralization

Cited:

Immunohistochemistry, Western Blot, Neutralization, Immunocytochemistry, ELISA Development, In vivo assay, Luminex Development

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # MP122E9
View all GM-CSF Primary Antibodies »

Product Specifications

Immunogen

Yeast derived recombinant mouse GM-CSF
Accession # P01587

Specificity

Detects mouse GM-CSF in ELISAs and Western blots. In Western blots, this antibody does not cross-react with recombinant human GM‑CSF.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Mouse GM-CSF Antibody (MP122E9)

Cell Proliferation Induced by GM‑CSF and Neutralization by Mouse GM‑CSF Antibody.

Cell Proliferation Induced by GM‑CSF and Neutralization by Mouse GM‑CSF Antibody.

Recombinant Mouse GM‑CSF (Catalog # 415-ML) stimulates proliferation in the DA3 mouse myeloma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse GM‑CSF (0.75 ng/mL) is neutralized (green line) by increasing concentrations of Mouse GM‑CSF Monoclonal Antibody (Catalog # MAB415). The ND50 is typically 0.03-0.09 µg/mL.
Detection of Mouse GM-CSF by In vivo assay

Detection of Mouse GM-CSF by In vivo assay

Effects of TRX on inflammatory cytokines in BALF from exposed to cigarette smoke and challenged with poly(I:C).(A) KC and (B) GM-CSF in BALF obtained 6 h and 3 days after poly(I:C) challenge. (C) Messenger RNA of GM-CSF in lung homogenates 3 days after poly(I:C) challenge in mice exposed to cigarette smoke treated with or without TRX. (D) Neutrophil and lymphocyte counts in BALF 3 days after poly(I:C) challenge in mice exposed to cigarette smoke treated with and without TRX or anti-GM-CSF antibody. Error bars represent standard deviation (SD) (A, B, and C, n = 3–4 per group; D and E, n = 5 per group); *p<0.05. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0079016), licensed under a CC-BY license. Not internally tested by R&D Systems.
Mouse GM-CSF ELISA Standard Curve

Mouse GM-CSF ELISA Standard Curve

Recombinant Mouse GM‑CSF (Catalog # 415-ML) was serially diluted and captured by Rat Anti-Mouse GM‑CSF Monoclonal Antibody (Catalog # MAB415) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse GM‑CSF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-415-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Mouse GM-CSF Antibody (MP122E9)

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Mouse GM-CSF (Catalog # 415-ML)
under non-reducing conditions only

Neutralization

Measured by its ability to neutralize GM‑CSF-induced proliferation in the DA3 mouse myeloma cell line. Ihle, J.N. et al. (1984) Advances in Viral Oncology. In G. Klein (eds): Raven Press, New York, NY. 4:95. The Neutralization Dose (ND50) is typically 0.03-0.09 µg/mL in the presence of 0.75 ng/mL Recombinant Mouse GM‑CSF.

Mouse GM-CSF Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Mouse GM‑CSF Biotinylated Antibody (Catalog # BAF415)
  • Standard: Recombinant Mouse GM-CSF Protein (Catalog # 415-ML)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using MAB415 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: GM-CSF

GM-CSF was initially characterized as a factor that can support the in vitro colony formation of granulocyte-macrophage progenitors. It is also a growth factor for erythroid, megakaryocyte, and eosinophil progenitors. GM-CSF is produced by a number of different cell types (including T cells, B cells, macrophages, mast cells, endothelial cells, fibroblasts, and adipocytes) in response to cytokine or inflammatory stimuli. On mature hematopoietic cells, GM-CSF is a survival factor for and activates the effector functions of granulocytes, monocytes/macrophages, and eosinophils (1, 2). GM-CSF promotes a Th1 biased immune response, angiogenesis, allergic inflammation, and the development of autoimmunity (3-5). It shows clinical effectiveness in ameliorating chemotherapy-induced neutropenia, and GM-CSF transfected tumor cells are utilized as cancer vaccines (6, 7). The 22 kDa glycosylated GM-CSF, similar to IL-3 and IL-5, is a cytokine with a core of four bundled alpha ‑helices (8-10). Mature mouse GM-CSF shares 49-54% amino acid sequence identity with canine, feline, human, and porcine GM-CSF and 69% with rat GM-CSF. GM‑CSF exerts its biological effects through a heterodimeric receptor complex composed of GM-CSF R alpha /CD116 and the signal transducing common beta chain (CD131) which is also a component of the high-affinity receptors for IL-3 and IL-5 (11, 12). In addition, GM-CSF binds a naturally occurring soluble form of GM-CSF R alpha (13). The activity of GM-CSF is species specific between human and mouse. Mouse GM-CSF is only weakly active on rat cells, although rat GM-CSF is fully active on mouse cells (14, 15).

References

  1. Martinez-Moczygemba, M. and D.P. Huston (2003) J. Allergy Clin. Immunol. 112:653.
  2. Barreda, D.R. et al. (2004) Dev. Comp. Immunol. 28:509.
  3. Eksioglu, E.A. et al. (2007) Exp. Hematol. 35:1163.
  4. Cao, Y. (2007) J. Clin. Invest. 117:2362.
  5. Fleetwood, A.J. et al. (2005) Crit. Rev. Immunol. 25:405.
  6. Heuser, M. et al. (2007) Semin. Hematol. 44:148.
  7. Hege, K.M. et al. (2006) Int. Rev. Immunol. 25:321.
  8. Kaushansky, K. et al. (1992) Biochemistry 31:1881.
  9. Diederichs, K. et al. (1991) Science 254:1779.
  10. Gough, N.M. et al. (1984) Nature 309:763.
  11. Onetto-Pothier, N. et al. (1990) Blood 75:59.
  12. Hayashida, K. et al. (1990) Proc. Natl. Acad. Sci. USA 87:9655.
  13. Pelley, J.L. et al. (2007) Exp. Hematol. 35:1483.
  14. Oaks, M.K. et al. (1995) J. Interferon Cytokine Res. 15:1095.
  15. Vandenabeele, P. et al. (1990) Lymphokine Res. 9:381.

Long Name

Granulocyte Macrophage Growth Factor

Alternate Names

CSF-2, CSF2, GMCSF, Molgramostim, Sargramostim

Entrez Gene IDs

1437 (Human); 12981 (Mouse); 116630 (Rat); 397208 (Porcine); 403923 (Canine); 493805 (Feline)

Gene Symbol

CSF2

UniProt

P01587

Additional GM-CSF Products

Product Documents for Mouse GM-CSF Antibody (MP122E9)

Certificate of Analysis

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Product Specific Notices for Mouse GM-CSF Antibody (MP122E9)

For research use only

Citations for Mouse GM-CSF Antibody (MP122E9)

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  • Mouse GM-CSF Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: DA3 mouse myeloma cell line
    Species: Mouse
    Verified Customer | Posted 09/30/2021
    Mouse GM-CSF Antibody (MP122E9) MAB415

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