Mouse Growth Hormone R/GHR Antibody

R&D Systems | Catalog # AF1360

R&D Systems

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Immunohistochemistry, Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Growth Hormone R/GHR Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Growth Hormone R/GHR
Thr25-Gln273
Accession # Q3UP14

Specificity

Detects mouse Growth Hormone R/GHR in direct ELISAs and Western blots. In Western blots, approximately 50% cross-reactivity with recombinant rat GHR is observed and approximately 20% cross-reactivity with recombinant human GHR is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse Growth Hormone R/GHR Antibody

Growth Hormone R/GHR antibody in Mouse Liver by Immunohistochemistry (IHC-Fr).

Growth Hormone R/GHR in Mouse Liver.

Growth Hormone R/GHR was detected in perfusion fixed frozen sections of mouse liver using Mouse Growth Hormone R/GHR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1360) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Detection of Growth Hormone R (GHR) by Immunocytochemistry/ Immunofluorescence

Detection of Growth Hormone R (GHR) by Immunocytochemistry/ Immunofluorescence

Effect of GH supplementation on ovary aging and the quality of aged oocytes. A Schedule of GH treatment and hormone administration to induce superovulation. B and C Morphological features of ovaries. Ovarian index = (ovary weight/body weight) × 100. D H&E staining of ovarian sections from young, aged, and GH + aged mice. PA, preantral follicle; AF, antral follicle; AT, atretic follicle; CL, corpus luteum. The bottom panel is the enlarged view of the box area in the top panel. Scale bar, 200 μm. E Numbers of follicles at the indicated stages in young (n = 3), aged (n = 3), and GH + aged (n = 3) mice. F Representative images of in vivo matured oocytes from young, aged, and GH + aged mice. Black arrows, oocytes with fragmentation. Scale bar, 80 μm. G Numbers of oocytes after superovulation in young (n = 10), aged (n = 10), and GH + aged (n = 10) mice. H and I Fragmentation and first polar body (PB1) extrusion rates in young (n = 39), aged (n = 35), and GH + aged (n = 42) oocytes. J Representative images of GHR in young, aged, and GH + aged oocytes. Scale bar, 20 μm. K and L Protein and mRNA levels of GHR revealed by immunofluorescence (n = 15) and real-time PCR (n = 25), respectively. M Cumulative number of pups over 12 weeks as a measure of the fertility of young (n = 6), aged (n = 6), and GH + aged (n = 6) mice. N Body weights of newborns (n = 24, 10, and 12 in the young, aged, and GH + aged groups, respectively) over 4 weeks. Data are means ± SEM of at least three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37386516), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse Growth Hormone R/GHR Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Perfusion fixed frozen sections of mouse kidney, liver, lung, thymus, and muscle

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse Growth Hormone R/GHR Fc Chimera (Catalog # 1360-GR)

Reviewed Applications

Read 1 review rated 4 using AF1360 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Growth Hormone R/GHR

Growth hormone (GH), also known as somatotropin, is a member of a family of growth factors that includes prolactin, placental lactogens, proliferins and somatolactin (1, 2). It is synthesized primarily by somatotropes in the anterior pituitary and is released as an endocrine hormone. Other cells and tissues, including lymphoid tissues, can also produce GH (3). GH is a pleiotropic molecule which can act directly or indirectly via IGF-I to regulate growth and metabolism as well as enhance T cell survival and thymic functions (1, 2, 4). GH exerts its biological actions by binding to the GH receptor (GHR) that is present in many cell types (1, 2). Mouse GHR cDNA encodes a 650 amino acid (aa) residue type I transmembrane protein with a 24 aa signal peptide, a 249 aa extracellular domain, a 24 aa transmembrane domain, and a 353 aa cytoplasmic domain (5). An alternatively spliced secreted isoform of mouse GHR also exists (6). This variant corresponds to the serum GH‑binding protein. Ligation of GHR by GH has been shown to result in receptor dimerization and activation of the JAK/STAT signaling cascade (7). The soluble GHBP has been shown to interfere with GH signaling by competing with the transmembrane receptor of GH. Alternatively, the GHBP has also been shown to enhance GH action by slowing GH clearance (8).

References

  1. Goffin, V. et al. (1996) Endocrine Rev. 17:385.
  2. Le Roith, D. et al. (2001) Endocrine Rev. 22:53.
  3. Clark, R. (1997) Endocr. Rev. 18:157.
  4. Welniak, L.A. et al. (2002) J. Leukoc. Biol. 71:381.
  5. Smith, W.C. et al. (1989) Mol. Endocrinol. 3:984.
  6. Edens, A. et al. (1994) Endocrinol. 135:2802.
  7. Carter-Su, C. et al. (1996) Annu. Rev. Physiol. 58:187.
  8. Postel-Vinay, M.C. and J. Finidori (1995) Eur. J. Endocrinol. 133:654.

Long Name

Growth Hormone Receptor

Alternate Names

GHR

Entrez Gene IDs

2690 (Human); 14600 (Mouse); 25235 (Rat)

Gene Symbol

GHR

UniProt

Q3UP14

Additional Growth Hormone R/GHR Products

Product Documents for Mouse Growth Hormone R/GHR Antibody

Certificate of Analysis

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Product Specific Notices for Mouse Growth Hormone R/GHR Antibody

For research use only

Citations for Mouse Growth Hormone R/GHR Antibody

Customer Reviews for Mouse Growth Hormone R/GHR Antibody (1)

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  • Mouse Growth Hormone R/GHR Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Liver tissue
    Species: Mouse
    Verified Customer | Posted 04/18/2019
    Mouse Growth Hormone R/GHR Antibody AF1360

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