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Mouse IL-10 Antibody

MAB417 35 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB417-SP
MAB417-100
MAB417-500
Mouse IL-10 Antibody in Data
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Product Details
Citations (35)
FAQs
Supplemental Products
Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse IL-10 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IL-10 in sandwich ELISAs and Western blots. In sandwich ELISAs, less than 8% cross-reactivity with recombinant rat IL-10 and 1% cross-reactivity with recombinant cotton rat IL‑10 is observed. In sandwich ELISAs, no cross-reactivity with recombinant human (rh) IL-10, recombinant mouse IL-10 R, rhIL‑10 R, recombinant canine IL-10, recombinant feline IL-10, or recombinant porcine IL-10 is observed.
Source
Monoclonal Rat IgG1 Clone # JES052A5
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant mouse IL-10
Accession # P18893
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Mouse IL-10 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Mouse IL‑10 Biotinylated Antibody (Catalog # BAF417)

Standard: Recombinant Mouse IL-10 Protein (Catalog # 417-ML)

Neutralization
Measured by its ability to neutralize IL‑10-induced proliferation in the MC/9‑2 mouse mast cell line. Thompson-Snipes, L.  et al. (1991) J. Exp. Med. 173:507. The Neutralization Dose (ND50) is typically 0.005-0.015 µg/mL in the presence of 2.5 ng/mL Recombinant Mouse IL‑10.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Recombinant Mouse IL‑10 by Western Blot. View Larger

Detection of Recombinant Mouse IL‑10 by Western Blot. Western blot shows 25 ng of Recombinant Mouse IL-10 (Catalog # 417-ML), Recombinant Human IL-10 (Catalog # 217-IL), Recombinant Rat IL-10 (Catalog # 522-RLB), and Recombinant Human IL-26/AK155 Monomer (Catalog # 1375-IL). PVDF Membrane was probed with 1 µg/mL of Rat Anti-Mouse IL-10 Monoclonal Antibody (Catalog # MAB417) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for IL-10 at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Neutralization Cell Proliferation Induced by IL‑10 and Neutralization by Mouse IL‑10 Antibody. View Larger

Cell Proliferation Induced by IL‑10 and Neutralization by Mouse IL‑10 Antibody. Recombinant Mouse IL-10 (Catalog # 417-ML) stimulates proliferation in the MC/9-2 mouse mast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-10 (2.5 ng/mL) is neutralized (green line) by increasing concen-trations of Rat Anti-Mouse IL-10 Monoclonal Antibody (Catalog # MAB417). The ND50is typically 0.005-0.015 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-10

Interleukin 10 (IL-10) is a pleiotropic cytokine that can exert either immunostimulatory or immunosuppressive effects on a variety of cell types. It is produced predominantly by Th2 lymphocytes and other activated lymphoid cells.

Long Name
Interleukin 10
Entrez Gene IDs
3586 (Human); 16153 (Mouse); 25325 (Rat); 397106 (Porcine); 403628 (Canine); 493683 (Feline); 100135553 (Guinea Pig); 2949786 (Viral)
Alternate Names
CSIF; CSIFMGC126450; Cytokine synthesis inhibitory factor; GVHDS; IL10; IL-10; IL10A; IL-10MGC126451; interleukin 10; interleukin-10; TGIF

Product Datasheets

Product Datasheet
COA
SDS

Citations for Mouse IL-10 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

35 Citations: Showing 1 - 10
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  1. I-alowCD11bhigh DC Regulates the Immune Response in the Eyes of Experimental Autoimmune Uveitis
    Authors: Y Zhao, J Wang, Z Min, L Peng, S Qi, W Lin
    Mediators Inflamm., 2020;2020(0):6947482.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  2. Protein Kinase A Catalytic Subunit Is a Molecular Switch that Promotes the Pro-tumoral Function of Macrophages
    Authors: YR Na, JW Kwon, DY Kim, H Chung, J Song, D Jung, H Quan, D Kim, JS Kim, YW Ju, W Han, HS Ryu, YS Lee, JJ Hong, SH Seok
    Cell Rep, 2020;31(6):107643.
    Species: Mouse
    Sample Types:
    Applications: IHC
  3. Perturbing chondroitin sulfate proteoglycan signaling through LAR and PTP? receptors promotes a beneficial inflammatory response following spinal cord injury
    Authors: S Dyck, H Kataria, A Alizadeh, KT Santhosh, B Lang, J Silver, S Karimi-Abd
    J Neuroinflammation, 2018;15(1):90.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Neutralization
  4. BMSCs pre-treatment ameliorates inflammation-related tissue destruction in LPS-induced rat DIC model
    Authors: B Wang, S Wu, Z Ma, T Wang, C Yang
    Cell Death Dis, 2018;9(10):1024.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Neutralization
  5. Electroacupuncture at ST-36 Protects Interstitial Cells of Cajal via Sustaining Heme Oxygenase-1 Positive M2 Macrophages in the Stomach of Diabetic Mice
    Authors: L Tian, S Song, B Zhu, S Liu
    Oxid Med Cell Longev, 2018;2018(0):3987134.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  6. CCR7 Deficiency Allows Accelerated Clearance of Chlamydia from the Female Reproductive Tract
    Authors: LX Li, JC Labuda, DM Imai, SM Griffey, SJ McSorley
    J. Immunol., 2017;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development (Capture)
  7. A critical role for IRF5 in regulating allergic airway inflammation
    Authors: AJ Byrne, M Weiss, SA Mathie, SA Walker, HL Eames, D Saliba, CM Lloyd, IA Udalova
    Mucosal Immunol, 2017;10(3):716-726.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: ELISA Development (Capture)
  8. Therapeutic reversal of food allergen sensitivity by mature retinoic acid-differentiated dendritic cell induction of LAG3(+)CD49b(-)Foxp3(-) regulatory T�cells
    Authors: W Dawicki, C Li, J Town, X Zhang, JR Gordon
    J. Allergy Clin. Immunol., 2017;139(5):1608-1620.e3.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development (Capture)
  9. IL-33 contributes to sepsis-induced long-term immunosuppression by expanding the regulatory T cell population
    Authors: DC Nascimento, PH Melo, AR Piñeros, RG Ferreira, DF Colón, PB Donate, FV Castanheir, A Gozzi, PG Czaikoski, W Niedbala, MC Borges, DS Zamboni, FY Liew, FQ Cunha, JC Alves-Filh
    Nat Commun, 2017;8(0):14919.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Functional Assay
  10. PI3Kgamma activates integrin alpha4 and promotes immune suppressive myeloid cell polarization during tumor progression
    Authors: JA Varner, P Foubert, MM Kaneda
    Cancer Immunol Res, 2017;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  11. Mesenchymal Stem Cell-Educated Macrophages Ameliorate LPS-Induced Systemic Response
    Authors: Yaoqin Hu
    Mediators Inflamm, 2016;2016(0):3735452.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  12. Enhanced immunoregulation of mesenchymal stem cells by IL-10-producing type 1 regulatory T cells in collagen-induced arthritis
    Authors: Jung-Yeon Lim
    Sci Rep, 2016;6(0):26851.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development (Capture)
  13. IL-33-mediated protection against experimental cerebral malaria is linked to induction of type 2 innate lymphoid cells, M2 macrophages and regulatory T cells.
    Authors: Besnard A, Guabiraba R, Niedbala W, Palomo J, Reverchon F, Shaw T, Couper K, Ryffel B, Liew F
    PLoS Pathog, 2015;11(2):e1004607.
    Species: Mouse
    Sample Types: In Vivo
  14. Myeloid cell TRAF3 regulates immune responses and inhibits inflammation and tumor development in mice.
    Authors: Lalani A, Moore C, Luo C, Kreider B, Liu Y, Morse H, Xie P
    J Immunol, 2015;194(1):334-48.
    Species: Mouse
    Sample Types: Serum
    Applications: ELISA Development
  15. Neuropathic pain is constitutively suppressed in early life by anti-inflammatory neuroimmune regulation.
    Authors: McKelvey R, Berta T, Old E, Ji R, Fitzgerald M
    J Neurosci, 2015;35(2):457-66.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  16. M2b macrophage elimination and improved resistance of mice with chronic alcohol consumption to opportunistic infections.
    Authors: Ohama H, Asai A, Ito I, Suzuki S, Kobayashi M, Higuchi K, Suzuki F
    Am J Pathol, 2015;185(2):420-31.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Functional Assay
  17. TLR9 ligands induce S100A8 in macrophages via a STAT3-dependent pathway which requires IL-10 and PGE2.
    Authors: Hsu, Kenneth, Chung, Yuen Min, Endoh, Yasumi, Geczy, Carolyn
    PLoS ONE, 2014;9(8):e103629.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  18. Delayed wound healing due to increased interleukin-10 expression in mice with lymphatic dysfunction.
    Authors: Kimura T, Sugaya M, Blauvelt A, Okochi H, Sato S
    J Leukoc Biol, 2013;94(1):137-45.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  19. Response patterns of cytokines/chemokines in two murine strains after irradiation.
    Authors: Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L
    Cytokine, 2012;58(2):169-77.
    Species: Mouse
    Sample Types: Plasma
    Applications: Luminex Development
  20. Interleukin-10 haplotype may predict survival and relapse in resected non-small cell lung cancer.
    Authors: Wang YC, Sung WW, Wu TC, Wang L, Chien WP, Cheng YW, Chen CY, Shieh SH, Lee H
    PLoS ONE, 2012;7(7):e39525.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  21. Treatment failure of a TLR-7 agonist occurs due to self-regulation of acute inflammation and can be overcome by IL-10 blockade.
    Authors: Lu H, Wagner WM, Gad E, Yang Y, Duan H, Amon LM, Van Denend N, Larson ER, Chang A, Tufvesson H, Disis ML
    J. Immunol., 2010;184(9):5360-7.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  22. Tr1 regulatory T cells induced by ConA pretreatment prevent mice from ConA-induced hepatitis.
    Authors: Ye F, Yan S, Xu L, Jiang Z, Liu N, Xiong S, Wang Y, Chu Y
    Immunol. Lett., 2009;122(2):198-207.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  23. ST2 deficient mice display a normal host defense against pulmonary infection with Mycobacterium tuberculosis.
    Authors: Wieland CW, van der Windt GJ, Florquin S, McKenzie AN, van der Poll T
    Microbes Infect., 2009;11(4):524-30.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  24. Tumor-specific Th17-polarized cells eradicate large established melanoma.
    Authors: Muranski P, Boni A, Antony PA, Cassard L, Irvine KR, Kaiser A, Paulos CM, Palmer DC, Touloukian CE, Ptak K, Gattinoni L, Wrzesinski C, Hinrichs CS, Kerstann KW, Feigenbaum L, Chan CC, Restifo NP
    Blood, 2008;112(2):362-73.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  25. CD14 contributes to pulmonary inflammation and mortality during murine tuberculosis.
    Authors: Wieland CW, van der Windt GJ, Wiersinga WJ, Florquin S, van der Poll T
    Immunology, 2008;125(2):272-9.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: ELISA Development
  26. CXCL12 (SDF-1alpha) suppresses ongoing experimental autoimmune encephalomyelitis by selecting antigen-specific regulatory T cells.
    Authors: Meiron M, Zohar Y, Anunu R, Wildbaum G, Karin N
    J. Exp. Med., 2008;205(11):2643-55.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  27. Induction of antigen-specific regulatory T lymphocytes by human dendritic cells expressing the glucocorticoid-induced leucine zipper.
    Authors: Hamdi H, Godot V, Maillot MC, Prejean MV, Cohen N, Krzysiek R, Lemoine FM, Zou W, Emilie D
    Blood, 2007;110(1):211-9.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  28. Relative importance of T-cell subsets in monocytotropic ehrlichiosis: a novel effector mechanism involved in Ehrlichia-induced immunopathology in murine ehrlichiosis.
    Authors: Ismail N, Crossley EC, Stevenson HL, Walker DH
    Infect. Immun., 2007;75(9):4608-20.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  29. Lack of in vitro and in vivo recognition of Francisella tularensis subspecies lipopolysaccharide by Toll-like receptors.
    Authors: Hajjar AM, Harvey MD, Shaffer SA, Goodlett DR, Sjostedt A, Edebro H, Forsman M, Bystrom M, Pelletier M, Wilson CB, Miller SI, Skerrett SJ, Ernst RK
    Infect. Immun., 2006;74(12):6730-8.
    Species: Human
    Sample Types: BALF
    Applications: ELISA Development
  30. Circulating cytokine/inhibitor profiles reshape the understanding of the SIRS/CARS continuum in sepsis and predict mortality.
    Authors: Osuchowski MF, Welch K, Siddiqui J, Remick DG
    J. Immunol., 2006;177(3):1967-74.
    Species: Mouse
    Sample Types: Plasma
    Applications: ELISA Development
  31. Severe sepsis exacerbates cell-mediated immunity in the lung due to an altered dendritic cell cytokine profile.
    Authors: Wen H, Hogaboam CM, Gauldie J, Kunkel SL
    Am. J. Pathol., 2006;168(6):1940-50.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: ELISA Development
  32. Sequential ELISA to profile multiple cytokines from small volumes.
    Authors: Osuchowski MF, Siddiqui J, Copeland S, Remick DG
    J. Immunol. Methods, 2005;302(1):172-81.
    Species: Mouse
    Sample Types: Plasma
    Applications: ELISA Development
  33. Selective macrophage suppression during sepsis.
    Authors: Ellaban E, Bolgos G, Remick D
    Cell. Immunol., 2004;231(1):103-11.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  34. Nonlymphoid peritoneal cells suppress the T cell response to Mls.
    Authors: Rosini L, Matlack R, Taylor J, Howell KF, Yeh K, Pennello A, Riggs JE
    Immunobiology, 2004;209(8):575-84.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  35. Attenuation of autoimmune disease in Fas-deficient mice by treatment with a cytotoxic benzodiazepine.
    Authors: Bednarski JJ, Warner RE, Rao T
    Arthritis Rheum., 2003;48(3):757-66.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ELISpot Development

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Reviews for Mouse IL-10 Antibody

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Mouse IL-10 Antibody
By Anonymous on 11/13/2020
Application: WB Sample Tested: Peritoneal macrophages Species: Mouse
Western Blot Mouse IL-10 Antibody MAB417