Mouse IL-2 R beta Antibody (130122)

Name: Mouse IL-2 R beta Antibody (130122)
Brand: R&D Systems
SKU: MAB5891
Price: 569 USD
Availability: InStock

R&D Systems | Catalog # MAB5891

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Product Specifications
Scientific Data
Applications
Flow Cytometry
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Mouse

Applications

Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG_2A Clone # 130122

View all IL-2 R beta Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse IL-2 R beta
Ala27-Glu240
Accession # P16297

Specificity

Detects mouse IL-2 R beta in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) IL‑2 R alpha, rhIL-2 R beta, recombinant mouse (rm) IL-2 R gamma, rhIL-15 R alpha, or rmIL-21 R is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG_2A

Scientific Data Images for Mouse IL-2 R beta Antibody (130122)

Detection of IL‑2 R beta in Mouse Splenocytes by Flow Cytometry.

Mouse splenocytes were stained with anti-mouse Integrin a2/CD49b APC-conjugated antibody and either (A) Rat Anti-Mouse IL-2 R beta Monoclonal Antibody (Catalog # MAB5891) or (B) Rat IgG_2AIsotype Control (Catalog # MAB006) followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B).

Applications for Mouse IL-2 R beta Antibody (130122)

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/10⁶ cells
Sample: Mouse splenocytes

Flow Cytometry Panel Builder

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Advanced Features

Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
Spillover Popups - Visualize the spectra of individual fluorochromes
Antigen Density Selector - Match fluorochrome brightness with antigen density

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Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: IL-2 R beta

IL‑2 R beta (IL-2 Receptor beta ; designated CD122) is a type I transmembrane protein that associates with IL-2 R alpha and the common gamma chain to form a high affinity heterotrimeric receptor complex for IL-2. IL‑2 R beta and the common gamma chain can also associate with IL-15 R alpha. IL-2 R beta is expressed by T, B, NK cells and monocyte/macrophages.