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Mouse Integrin  alpha 7 Fluorescein-conjugated Antibody

Catalog #: FAB3518F
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB3518F

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Detection of Integrin alpha 7 in C2C12 Mouse Cell Line by Flow Cytometry.
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Citations (2)
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Mouse Integrin  alpha 7 Fluorescein-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Integrin alpha 7 in direct ELISAs. Reactivity with other Integrin alpha 7 isoforms was not tested. In direct ELISAs, no cross-reactivity with recombinant mouse Integrin alpha 2, alpha 3, alpha 4, alpha 5, alpha 9, alpha E, alpha X, recombinant human Integrin alpha L, or alpha 11 is observed.
Source
Monoclonal Rat IgG2B Clone # 334908
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant mouse Integrin  alpha 7
Phe34-Pro1036
Accession # NP_032424
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Fluorescein (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Integrin a7 antibody in C2C12 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of Integrin alpha 7 in C2C12 Mouse Cell Line by Flow Cytometry. C2C12 mouse myoblast cell line was stained with Rat Anti-Mouse Integrin a7 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB3518F, filled histogram) or isotype control antibody (Catalog # IC013F, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Integrin alpha 7

Integrin alpha 7 is a transmembrane glycoprotein that forms heterodimers with Integrin  beta 1 to bind Laminin 1.

Entrez Gene IDs
3679 (Human); 16404 (Mouse); 81008 (Rat); 102130741 (Cynomolgus Monkey)
Alternate Names
FLJ25220; integrin alpha 7 chain; Integrin alpha 7; integrin alpha-7; integrin, alpha 7; ITGA7

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Citations for Mouse Integrin  alpha 7 Fluorescein-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. A role for Galgt1 in skeletal muscle regeneration.
    Authors: Singhal N, Martin P
    Skelet Muscle, 2015-01-27;5(0):3.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Mechano-growth factor peptide, the COOH terminus of unprocessed insulin-like growth factor 1, has no apparent effect on myoblasts or primary muscle stem cells.
    Authors: Fornaro, Mara, Hinken, Aaron C, Needle, Saul, Hu, Erding, Trendelenburg, Anne-Ulr, Mayer, Angelika, Rosenstiel, Antonia, Chang, Calvin, Meier, Viktor, Billin, Andrew N, Becherer, J David, Brace, Arthur D, Evans, William, Glass, David J, Russell, Alan J
    Am J Physiol Endocrinol Metab, 2013-11-19;306(2):E150-6.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Functional Assay

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