Mouse Integrin alpha M/CD11b Alexa Fluor® 700-conjugated Antibody

FAB1124N Datasheet / COA / SDS

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FAB1124N-100
FAB1124N-025

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Detection of Integrin alpha M/CD11b in Mouse Splenocytes by Flow Cytometry.

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Mouse Integrin alpha M/CD11b Alexa Fluor® 700-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Integrin alpha M/CD11b. Cross-reaction with human Integrin alpha M has been reported (1, 2).

Source

Monoclonal Rat IgG_2B Clone # M1/70

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Con A-activated C57BL/10 splenocytes

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Alexa Fluor 700

Applications

Recommended Concentration

Sample

Flow Cytometry

5 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry

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Detection of Integrin alpha M/CD11b in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse Integrin alpha M/CD11b Alexa Fluor® 700‑conjugated Monoclonal Antibody (Catalog # FAB1124N) and Rat Anti-Mouse F4/80/EMR1 PE‑conjugated Monoclonal Antibody (Catalog # FAB5580P). Quadrant markers were set based on control antibody staining (Catalog # IC013N). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD11b/Integrin alpha M

The Integrin family proteins are heterodimeric transmembrane receptors composed of an alpha and a beta subunit. The Integrin alpha M subunit, also known as MAC-1 alpha subunit or CD11b, combines with the Integrin beta 2 subunit (CD18) to form the non-covalent heterodimer Integrin alpha M/ beta 2, also known as MAC-1 and Complement Receptor type 3 (CR3). Integrin alpha M/ beta 2 is expressed on granulocytes, macrophages, dendritic cells and natural killer cells. Upon activation, alpha M/ beta 2 can bind several ligands (including ICAM-1, fibrinogen, and the C3 complement fragment, C3bi) to mediate phagocyte adhesion, migration and ingestion of complement-opsonized particles.

References

Beller, D.J. et al. (1982) J. Exp. Med. 156:1000.
Ault, K.A. and T.A. Springer (1981) J. Immunol. 126:359.

Entrez Gene IDs

3684 (Human); 16409 (Mouse)

Alternate Names

antigen CD11b (p170); CD11 antigen-like family member B; CD11b antigen; CD11b; CD11Bintegrin, alpha M (complement component receptor 3, alpha; also known as CD11b(p170), macrophage antigen alpha polypeptide); Cell surface glycoprotein MAC-1 subunit alpha; CR-3 alpha chain; CR3AMGC117044; Integrin alpha M; integrin alpha-M; integrin, alpha M (complement component 3 receptor 3 subunit); ITGAM; Leukocyte adhesion receptor MO1; MAC-1; MAC1A; macrophage antigen alpha polypeptide; MO1A; neutrophil adherence receptor alpha-M subunit; Neutrophil adherence receptor; SLEB6