Mouse Kallikrein 1 Antibody

Catalog #: AF7928 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF7928
AF7928-SP

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Detection of Mouse Kallikrein 1 by Western Blot.

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Mouse Kallikrein 1 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Kallikrein 1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human Kallikrein 1, recombinant mouse (rm) Kallikrein 5 and rmKallikrein 7 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Kallikrein 5 precursor/Kallikrein 1
Val23-Arg67(Kallikrein 5 precursor), Ile25-Asp261(Kallikrein 1)
Accession # NP_081082, P15947

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.25 µg/mL

See below

Immunohistochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Mouse Kallikrein 1 by Western Blot. Western blot shows lysates of mouse pancreas tissue and mouse small intestine. PVDF membrane was probed with 0.25 µg/mL of Sheep Anti-Mouse Kallikrein 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7928) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Kallikrein 1 at approximately 27-30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

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Kallikrein 1 in Mouse Kidney. Kallikrein 1 was detected in perfusion fixed frozen sections of mouse kidney using Sheep Anti-Mouse Kallikrein 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7928) at 1.5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to tubules. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Kallikrein 1

The kallikreins are a family of trypsin‑like serine proteases, many of which are associated with a variety of cancers. Kallikrein 1 (KLK1) also known as tissue kallikrein and urinary kallikrein is a 25 - 30 kDa member of peptidase S1 family of proteins. At least two isoforms are predicted. An important physiological function of KLK1 is the cleavage of kininogen to release a vasoactive kinin peptide, bradykinin (for rodent KLK1) or lysyl‑bradykinin (for human KLK1). Kinins regulate vasodilation, blood pressure reduction, smooth muscle relaxation and contraction, pain induction and inflammation. Over aa 25-261, mouse KLK1 shares 64% aa identity with human KLK1.

Entrez Gene IDs

3816 (Human); 16612 (Mouse)

Alternate Names

EC 3.4.21; EC 3.4.21.35; glandular kallikrein 1; hK1; Kallikrein 1; kallikrein 1, renal/pancreas/salivary; kallikrein serine protease 1; kallikrein-1; Kidney/pancreas/salivary gland kallikrein; KLK1; Klk6; KLKR; Tissue kallikrein