Mouse Ly-6G (Gr-1) Antibody (1A8)

R&D Systems | Catalog # MAB10371

R&D Systems
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Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse, Transgenic Mouse

Applications

Validated:

Flow Cytometry, CyTOF-ready

Cited:

Immunohistochemistry, Neutralization, Flow Cytometry, In vivo assay, Neutrophil Depletion

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2B Clone # 1A8
View all Ly-6G (Gr-1) Primary Antibodies »

Product Specifications

Immunogen

Ly-6G transfected EL-4J cell line
Accession # P35461

Specificity

Detects mouse Ly-6G in Flow Cytometry.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for Mouse Ly-6G (Gr-1) Antibody (1A8)

Detection of Ly-6G (GR-1) antibody in Mouse Bone Marrow Cells antibody by Flow Cytometry

Detection of Ly-6G (GR-1) in Mouse Bone Marrow Cells by Flow Cytometry

Mouse bone marrow cells were stained with either (A) Rat Anti-Mouse Ly-6g (GR-1) Monoclonal Antibody (Catalog # MAB10371) or (B) Rat IgG2A Isotype Control (Catalog # MAB006) followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B) and Rat Anti-Mouse B220/CD45R APC-conjugated Monoclonal Antibody (Catalog # FAB1217A).View our protocol for Staining Membrane-associated Proteins.
Detection of Ly-6G (Gr-1) by Flow Cytometry

Detection of Ly-6G (Gr-1) by Flow Cytometry

CCL24 i.p. injection recruits neutrophils and monocytes. (A) Outline of the experimental procedure. (B–F) Identification of peritoneal immune populations by scRNA-seq. (B) Uniform manifold approximation and projection (UMAP) plot of all cells that passed QC, clustered by the Louvain algorithm. (C) Heatmap of the top four gene markers for each cluster. Expression levels are scaled, and the number of cells in each column is downsampled to 500. (D) Stacked bar plot showing the relative abundance of each cell type, per treatment. (E) Violin plots of CCL24 expression levels by cell type. (F) Violin plots of CCL24 expression levels by cell type and treatment. (G–J) Identification of peritoneal immune populations by flow-cytometry. (G) Representative scatter plots of CD11b+ cells. (H–J) Quantification of relative change in cell counts for monocytes (H), neutrophils (I), and T cells (J). Data are represented as mean ± SEM (n = 4–10). * p < 0.05; ** p < 0.01. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/38334601), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse Ly-6G (Gr-1) Antibody (1A8)

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: Mouse bone marrow cells

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Ly-6G (Gr-1)

The myeloid differentiation antigen Gr-1, also known as Ly-6G, is a member of the Ly-6 family (1). The 1A8 antibody binds to Ly-6G while the RB6‑8C5 antibody also reacts weakly with Ly-6C-transfected EL4 cells (2). Ly-6G is a 21-25 kDa glycosyolphosphatidylinositol-anchored protein that is predominatly expressed in granulocytes in bone marrow.

References

  1. Spangrude, G.J. et al. (1988) Science 241:58.
  2. Fleming, T.J. et al. (1993) J. Immunol. 151:2399.

Long Name

A Myeloid Differentiation Antigen

Alternate Names

Gr-1

Entrez Gene IDs

546644 (Mouse)

Gene Symbol

LY6G

UniProt

P35461

Additional Ly-6G (Gr-1) Products

Product Documents for Mouse Ly-6G (Gr-1) Antibody (1A8)

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Product Specific Notices for Mouse Ly-6G (Gr-1) Antibody (1A8)

For research use only

Citations for Mouse Ly-6G (Gr-1) Antibody (1A8)

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