Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Rat IgG1 Clone # 579511
View all MARCO Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse MARCO
Gln70-Ser518
Accession # Q60754

Specificity

Detects mouse MARCO in direct ELISAs.

Clonality

Monoclonal

Host

Rat

Isotype

IgG1

Scientific Data Images for Mouse MARCO APC‑conjugated Antibody

Detection of MARCO antibody in Mouse Splenocytes antibody by Flow Cytometry.

Detection of MARCO in Mouse Splenocytes by Flow Cytometry.

Mouse splenocytes were stained with Rat Anti-Mouse MARCO APC-conjugated Monoclonal Antibody (Catalog # FAB2956A, filled histogram) or isotype control antibody (Catalog # IC005A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Detection of MARCO antibody in J774 Mouse Cell Line antibody by Flow Cytometry.

Detection of MARCO in J774 Mouse Cell Line by Flow Cytometry.

J774 mouse monocyte-macrophage cell line, resting (open histogram), or treated with 500 ng/ml LPS for 3 days (filled histogram) were stained with Rat Anti-Mouse MARCO APC-conjugated Monoclonal Antibody (Catalog # FAB2956A). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse MARCO APC‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: Mouse splenocytes or J774 mouse cell line treated with LPS

Reviewed Applications

Read 1 review rated 4 using FAB2956A in the following applications:

Spectra Viewer

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: MARCO

Mouse MARCO is a type II transmembrane glycoprotein belonging to the class A scavenger receptor family. It is constitutively expressed in subsets of macrophages found in the marginal zone of the spleen, the peritoneum, and the medullary cord of lymph nodes. The extracellular domains of MARCO form a 220 kDa disulfide-linked homotrimer on the cell surface. MARCO binds both gram positive and gram negative bacteria, as well as oxidized low-density lipoprotein. The amino acid sequence of mouse MARCO extracellular domain is 69% identical to that of human MARCO.

Long Name

Macrophage Receptor with Collagenous Structure/Scavenger Receptor, Class A, Member 2

Alternate Names

SCARA2

Entrez Gene IDs

8685 (Human); 17167 (Mouse)

Gene Symbol

MARCO

UniProt

Q60754

Additional MARCO Products

Product Documents for Mouse MARCO APC‑conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse MARCO APC‑conjugated Antibody

For research use only

Citations for Mouse MARCO APC‑conjugated Antibody

Customer Reviews for Mouse MARCO APC‑conjugated Antibody (1)

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  • Mouse MARCO APC-conjugated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: 293T human embryonic kidney cell line
    Species: Human
    Verified Customer | Posted 12/07/2018
    Binds really nicely to muMarco transiently expressing cells, does not bind to untransfected cells.
    Mouse MARCO APC‑conjugated Antibody FAB2956A

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