Mouse Meteorin-like/METRNL Antibody

R&D Systems | Catalog # AF6679

R&D Systems

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Immunohistochemistry

Cited:

Western Blot, Chromatin Immunoprecipitation (ChIP)

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all Meteorin-like/METRNL Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant mouse Meteorin-like/METRNL
Accession # Q8VE43

Specificity

Detects mouse Meteorin-like/METRNL in direct ELISAs. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse Meteorin is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Mouse Meteorin-like/METRNL Antibody

Meteorin-like/METRNL antibody in Mouse Embryo by Immunohistochemistry (IHC-Fr).

Meteorin‑like/METRNL in Mouse Embryo.

Meteorin-like/METRNL was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Sheep Anti-Mouse Meteorin-like/METRNL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6679) at 0.6 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to muscle cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Applications for Mouse Meteorin-like/METRNL Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed frozen sections of mouse embryo (13 d.p.c.)

Reviewed Applications

Read 1 review rated 5 using AF6679 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Meteorin-like/METRNL

Meteorin-like protein (METRNL) is a 311 amino acid (aa) protein that shows 45% homology with Meteorin (in mouse). Meteorin itself is a secreted 30 kDa protein that is expressed in glial and neuronal cells and their precursors during development and in the adult (1‑3). In the adult Meteorin is primarily expressed by astrocytes (3). Meteorin promotes neurite outgrowth, glial cell differentiation, and neuronal survival following excitotoxic injury (2‑5). It also inhibits sprouting angiogenesis and promotes blood vessel maturation (1). Mouse Meteorin‑like protein is synthesized with a 45 amino acid (aa) signal peptide and is selectively expressed in the otic vesicle during inner ear development (6, 7). Mature mouse Meteorin‑like protein shares 79% and 98% aa sequence identity with human and rat Meteorin‑like protein, respectively. Alternate splicing generates an additional isoform that lacks the N‑terminal 82 amino acids including the signal peptide. R&D Systems in‑house testing indicates that Meteorin‑like protein enhances the outgrowth of neurites from cultured mouse cortical neurons. The promoter region of the METRNL gene contains a recognition site for the Pax2, Pax5, and Pax8 transcription factors, and Pax2 binds specifically to this region (7). METRNL is the only gene found within the terminus of the human chromosome 17 q‑arm which can be deleted in the rare Ring 17 syndrome (8).

References

  1. Park, J.A. et al. (2008) Glia 56:247.
  2. Jorgensen, J.R. et al. (2009) J. Mol. Neurosci. 39:104.
  3. Lee, H.S. et al. (2010) J. Cell Sci. 123:1959.
  4. Nishino, J. et al. (2004) EMBO J. 23:1998.
  5. Jorgensen, J.R. et al. (2011) Neurobiol. Dis. 41:160.
  6. Accession # Q8VE43.
  7. Ramialison, M. et al. (2008) Genome Biol. 9:R145.
  8. Surace, C. et al. (2009) Clin. Genet. 76:256.

Alternate Names

Meteorin like, METRNL

Entrez Gene IDs

284207 (Human); 210029 (Mouse); 316842 (Rat)

Gene Symbol

METRNL

UniProt

Q8VE43

Additional Meteorin-like/METRNL Products

Product Documents for Mouse Meteorin-like/METRNL Antibody

Certificate of Analysis

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Product Specific Notices for Mouse Meteorin-like/METRNL Antibody

For research use only

Related Research Areas

Glial Lineage Markers

Citations for Mouse Meteorin-like/METRNL Antibody

Customer Reviews for Mouse Meteorin-like/METRNL Antibody (1)

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  • Mouse Meteorin-like/METRNL in mouse tissue
    Name: Aranzazu Pintor
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Kidney tissue
    Species: Mouse
    Verified Customer | Posted 10/01/2026
    The image shows specific and well-defined staining for METRNL in the renal cortex of a mouse with acute kidney injury (AKI). The positive signal is localized primarily in tubular epithelial cells, exhibiting a heterogeneous distribution.
    We have used the Mouse Meteorin-like/METRNL Antibody in mouse kidney samples with acute kidney injury (AKI), using a 1:100 dilution for immunohistochemistry. The antibody demonstrated specific and well-defined staining, particularly in the renal cortex, as shown in the attached image. The signal clearly identifies METRNL-positive areas, providing good contrast with the surrounding tissue and exhibiting a low level of nonspecific staining. In the context of AKI, the observed staining pattern is especially noteworthy, as METRNL has been associated with inflammatory, metabolic, and tissue injury response processes. In our sample, the cortical staining reveals a localized distribution of the protein within renal structures affected by acute injury, facilitating the study of its potential role in the renal tissue response to damage.
    Mouse Meteorin-like/METRNL Antibody AF6679

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