Mouse NG2/MCSP Antibody (546930)

R&D Systems | Catalog # MAB6689

R&D Systems

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Immunohistochemistry

Cited:

Immunohistochemistry, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG1 Clone # 546930
View all NG2/MCSP Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse NG2/MCSP
Leu1587-Ser2229
Accession # Q8VHY0

Specificity

Detects mouse NG2/MCSP in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human NG2/MCSP/CSPG4 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG1

Scientific Data Images for Mouse NG2/MCSP Antibody (546930)

NG2/MCSP antibody in Mouse Brain by Immunohistochemistry (IHC-Fr).

NG2/MCSP in Mouse Brain.

NG2/MCSP was detected in perfusion fixed frozen sections of mouse brain (cortex) using Rat Anti-Mouse NG2/MCSP Monoclonal Antibody (Catalog # MAB6689) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hemotoxylin (blue). Specific staining was localized to glial cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Applications for Mouse NG2/MCSP Antibody (546930)

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Perfusion fixed frozen sections of mouse brain (cortex)

Reviewed Applications

Read 2 reviews rated 3.5 using MAB6689 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: NG2/MCSP

NG2, also called MCSP (melanoma-associated chondroitin sulfate proteoglycan), CSPG4, or AN2, is a 400-500 kDa proteoglycan found on multiple cell types, including immature oligodendrocytes, osteoblasts, chondroblasts, and pericytes.  It interacts with integrin alpha 3 beta 1, galectin 3, MMP16, collagen VI and PDGFR alpha.  Mature mouse NG2 is a 2298 amino acid (aa) type I transmembrane glycoprotein.  The extracellular region is 2200 aa in length (aa 30-2229), while the cytoplasmic domain is 76 aa in size. The glycoprotein component of NG2 is approximately 300-315 kDa in size.  Proteolytic cleavage generates a 290 and 275 kDa form (excluding the chondroitin sulfate moiety).  There are two splice variants.  One shows a 38 aa substitution for aa 1657-2327, while another shows an alternate start site at Met1667.  Over aa 31-420, mouse NG2 shares 95% and 80% aa identity with rat and human NG2, respectively.    

Long Name

Chondroitin Sulfate Proteoglycan NG2/Melanoma Associated Chondroitin Sulfate Proteoglycan

Alternate Names

AN2, CSPG4, HMW-MAA, MCSP, MCSPG, MEL-CSPG

Entrez Gene IDs

1464 (Human); 121021 (Mouse); 81651 (Rat)

Gene Symbol

CSPG4

UniProt

Q8VHY0

Additional NG2/MCSP Products

Product Documents for Mouse NG2/MCSP Antibody (546930)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse NG2/MCSP Antibody (546930)

For research use only

Citations for Mouse NG2/MCSP Antibody (546930)

Customer Reviews for Mouse NG2/MCSP Antibody (546930) (2)

3.5 out of 5
2 Customer Ratings
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Customer Images

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  • Mouse NG2/MCSP Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Brain tissue
    Species: Mouse
    Verified Customer | Posted 01/13/2026
    postnatal day 7, mouse brain, cerebral cortex, 2%PFA fixed frozen section, 2.5 ug/ml
    Mouse NG2/MCSP Antibody (546930) MAB6689
  • Mouse NG2/MCSP Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Brain tissue
    Species: Mouse
    Verified Customer | Posted 11/02/2016
    Mouse NG2/MCSP Antibody (546930) MAB6689

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Protocols

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