Mouse PLTP Alexa Fluor® 750-conjugated Antibody

Catalog #: AF4918S Datasheet / COA / SDS

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AF4918S-100UG

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Mouse PLTP Alexa Fluor® 750-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse PLTP in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 50% cross-reactivity with recombinant human PLTP is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse PLTP
Glu18-Ala493 (Asp32Glu)
Accession # P55065

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 750 (Excitation= 749 nm, Emission= 775 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: PLTP

PLTP (phospholipid transfer protein) is a 65-80 kDa, secreted glycoprotein member of the BPI/LBP family of proteins. It is expressed by multiple cell types, circulates bound to HDL, and mediates the transfer of phospholipids and cholesterol from apoB-containing lipoproteins to HDL. Mature mouse PLTP is 476 amino acids (aa) in length. It contains an N-terminal lipid transfer domain (aa 21-239), a C-terminal HDL-binding domain (aa 263-463), and one essential intrachain disulfide bond (Cys146‑Cys185). There are three potential PLTP splice variants. One shows a deletion of aa 148-493, a second shows a deletion of aa 111-162, and a third shows a deletion of aa 14-33 plus a two aa substitution for aa 184-493. Over aa 18-493, mouse PLTP shares 92% and 83% aa identity with rat and human PLTP, respectively.

Long Name

Phospholipid Transfer Protein

Entrez Gene IDs

5360 (Human); 18830 (Mouse); 296371 (Rat)

Alternate Names

HDLCQ9; Lipid transfer protein II; phospholipid transfer protein; PLTP