Mouse Podoplanin Antibody

Catalog #: MAB11608 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB11608-100
MAB11608-SP

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Detection of Mouse Podoplanin by Western Blot.

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Mouse Podoplanin Antibody Summary

Species Reactivity

Mouse

Specificity

Detects recombinant mouse Podoplanin in Direct ELISA

Source

Monoclonal Rat IgG₁ Clone # 1090106

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line, NS0-derived mouse Podoplanin
Gln21-Lys133
Accession # Q62011

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Mouse lung tissue

Immunohistochemistry

3-25 µg/mL

Immersion fixed paraffin-embedded sections of mouse kidney and lung

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Mouse Podoplanin by Western Blot. Western Blot shows lysates of mouse lung tissue. PVDF membrane was probed with 1 µg/ml of Rat Anti-Mouse Podoplanin Monoclonal Antibody (Catalog # MAB11608) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Podoplanin at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Immunohistochemistry

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Detection of Podoplanin in Mouse Kidney. Podoplanin was detected in immersion fixed paraffin-embedded sections of mouse kidney using Rat Anti-Mouse Podoplanin Monoclonal Antibody (Catalog # mab11608) at 5 µg/ml overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using the HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005) and counterstained with hematoxylin (blue). Specific staining was localized to the cell membrane of podocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunohistochemistry

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Detection of Podpalanin in Mouse Lung. Podoplanin was detected in immersion fixed paraffin-embedded sections of mouse lung using Rat Anti-Mouse Podoplanin Monoclonal Antibody (Catalog # mab11608) at 5 µg/ml overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using the HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005) and counterstained with hematoxylin (blue). Specific staining was localized to the cell membrane of alveoli. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute lyophilized material at 0.2 mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Podoplanin

Podoplanin, also known as T1 alpha (T1 alpha ), is a mucin type transmembrane glycoprotein with extensive O-glycosylation. It is specifically expressed by lymphatic endothelial cells but not blood vascular endothelial cells. In addition, non-endothelial cells in numerous normal tissues also express the protein. Within the region used as the immunogen, mouse Podoplanin shares 73.5% and 29% amino acid sequence homology with rat and human Podoplanin, respectively.

Long Name

Lung Type-I Cell Membrane-associated Glycoprotein, Isoform A

Entrez Gene IDs

10630 (Human); 14726 (Mouse); 54320 (Rat)

Alternate Names

36-KD; Aggrus; Gp36; Gp38; GP40; HT1A-1; hT1alpha-1; hT1alpha-2; lung type I cell membrane associated glycoprotein; lung type-I cell membrane-associated glycoprotein (T1A-2); OTS 8; OTS8; PA2.26 antigen; PDPN; Podoplanin; RANDAM-2; T1A; T1A-2; T1-alpha