Goat anti-IgG VisUCyte Secondary Antibody [HRP Polymer]

R&D Systems | Catalog # VC002

R&D Systems
Discontinued Product
VC002 has been discontinued. View all IgG products.

Key Product Details

Species Reactivity

Mouse, Rabbit

Applications

Immunohistochemistry

Label

HRP Polymer

Antibody Source

Polyclonal Goat IgG
View all IgG Secondary Antibodies »

Product Specifications

Immunogen

Mouse and Rabbit IgG

Specificity

Detects heavy ( gamma ) chains on mouse and rabbit IgG and light chains on all mouse and rabbit immunoglobulins, based on IEP. No cross-reactivity is observed with non-immunoglobulin mouse or rabbit serum proteins or serum proteins from bovine, horse, human, pig or rat, based on IEP. VisUCyte™ HRP Polymer provides high sensitivity with low background in IHC.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Goat anti-IgG VisUCyte Secondary Antibody [HRP Polymer]

Detection of FABP1/L-FABP in Human Kidney.

Detection of FABP1/L-FABP in Human Kidney.

FABP1/L-FABP was detected in paraffin-embedded sections of human kidney using Mouse Anti-Human FABP1/L-FABP Monoclonal Antibody (Catalog # MAB2964) at 1 µg/mL for 1 hour at room temperature followed by incubation for 30 minutes at room temperature with Anti-Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody (Catalog # VC002). Tissue was stained with DAB (brown color) and counterstained with hematoxylin (blue color).
Detection of Phospho-p38 (T180/Y182) in Mouse Embryo (13 d.p.c.).

Detection of Phospho-p38 (T180/Y182) in Mouse Embryo (13 d.p.c.).

Phospho-p38 (T180/Y182) was detected in frozen sections of mouse embryo (13 d.p.c., cross-section through the gut) using Rabbit Anti-Human/Mouse/Rat Phospho-p38 MAP Kinase (T180/Y182) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF869) at 1 µg/mL for 1 hour at room temperature followed by incubation for 30 minutes at room temperature with Anti-Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody (Catalog # VC002). Tissue was stained with DAB (brown color) and counterstained with hematoxylin (blue color).

Applications for Goat anti-IgG VisUCyte Secondary Antibody [HRP Polymer]

Application
Recommended Usage

Immunohistochemistry

Prediluted, ready to use with formalin fixed paraffin-embedded and frozen sections. Compatible with most commonly used fixatives.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Supplied as a solution in PBS with preservative.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

6 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG

VisUCyte™ HRP Polymer is a biotin-free detection reagent which overcomes problems of avidin-biotin detection chemistry, such as endogenous biotin staining and the need to do additional quenching of endogenous biotin and avidin present in some tissues. With VisUCyte HRP Polymer it is possible to achieve specific staining in cells and tissue much faster than the conventional avidin-biotin-HRP procedure. Due the high sensitivity of VisUCyte™ HRP Polymer, the working concentration of primary antibodies may be 2-3 times lower compared to the conventional avidin-biotin-HRP protocol.

Long Name

Immunoglobulin G

Alternate Names

Immunoglobulin G, ImmunoglobulinG

Additional IgG Products

Product Documents for Goat anti-IgG VisUCyte Secondary Antibody [HRP Polymer]

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Goat anti-IgG VisUCyte Secondary Antibody [HRP Polymer]

For research use only

Citations for Goat anti-IgG VisUCyte Secondary Antibody [HRP Polymer]

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Protocols

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FAQs

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