Key Product Details

Species Reactivity

Validated:

Mouse, Rat

Cited:

Mouse

Applications

Validated:

Immunocytochemistry

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2B Clone # 228208
View all Follistatin-like 1/FSTL1 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Follistatin-like 1/FSTL1
Glu19-Ile306
Accession # NP_032073

Specificity

Detects mouse Follistatin-like 1/FSTL1 in direct ELISAs.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for Mouse/Rat Follistatin‑like 1/FSTL1 Antibody

Follistatin-like 1/FSTL1 antibody in PC-12 Rat Cell Line by Immunocytochemistry (ICC).

Follistatin-like 1/FSTL1 in PC‑12 Rat Cell Line.

Follistatin-like 1/FSTL1 was detected in immersion fixed PC-12 rat adrenal pheochromocytoma cell line using Rat Anti-Mouse/Rat Follistatin-like 1/FSTL1 Mono-clonal Antibody (Catalog # MAB17381) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the Northern-Lights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Mouse/Rat Follistatin‑like 1/FSTL1 Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed PC-12 rat adrenal pheochromocytoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Follistatin-like 1/FSTL1

Follistatin-Like 1 (FSTL1), also known as FSTL1 and follistatin-related protein (FRP), is a secreted protein that contains a follistatin-like domain, a Kazal-like domain and a von Willebrand factor C domain. Human FSTL1 shares 92% amino acid sequence identity with the mouse FSTL1, also known as TSC-36.

Alternate Names

Flik, Follistatin-related Protein, FRP, FSL1, FSTL1, TSC-36

Entrez Gene IDs

11167 (Human); 14314 (Mouse); 79210 (Rat)

Gene Symbol

FSTL1

UniProt

NP_032073

Additional Follistatin-like 1/FSTL1 Products

Product Documents for Mouse/Rat Follistatin‑like 1/FSTL1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse/Rat Follistatin‑like 1/FSTL1 Antibody

For research use only

Citations for Mouse/Rat Follistatin‑like 1/FSTL1 Antibody

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