Mouse S100A4 Antibody

Detection of S100A4 in Mouse Lung via seqIF™ staining on COMET™ S100A4 was detected in perfusion fixed paraffin-embedded sections of mouse Lung using Rabbit Anti-Mouse S100A4, Monoclonal Antibody (Catalog # MAB11727) at 0.1ug/mL at 37° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ Plus 647 Goat anti-Rabbit IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the nucleus and cytoplasm. Protocol available in COMET™ Panel Builder.

Detection of S100A4 in Mouse Spleen via seqIF™ staining on COMET™​ S100A4 was detected in immersion fixed paraffin-embedded sections of mouse Spleen using Rabbit Anti-Mouse S100A4, Monoclonal Antibody (Catalog # MAB11727) at 0.1ug/mL at 37° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ Plus 647 Goat anti-Rabbit IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the nucleus and cytoplasm. Protocol available in COMET™ Panel Builder.

Detection of S100A4 in Mouse Spleen. S100A4 was detected in perfusion fixed paraffin-embedded sections of mouse spleen using Rabbit Anti-Mouse S100A4 Monoclonal Antibody (Catalog # MAB11727) at 0.05 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003) or the HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the nucleus and cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Detection of S100A4 in Mouse Thymus. S100A4 was detected in perfusion fixed paraffin-embedded sections of mouse thymus using Rabbit Anti-Mouse S100A4 Monoclonal Antibody (Catalog # MAB11727) at 0.05 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003) or the HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the nucleus and cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Detection of S100A4 in RAW264.7 cells S100A4 was detected in Immersion fixed RAW264.7 cells (Positive) and low in Neuro2A cells (Low expression) using Rabbit Anti-Mouse S100A4 Monoclonal Antibody (Catalog # MAB11727) at 3 µg/ml for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to the nucleus and cytoplasm of RAW264.7 cells. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of Mouse S100A4 by Western Blot. Western Blot shows lysates of NIH‑3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 0.05 µg/ml of Rabbit Anti-Mouse S100A4 Monoclonal Antibody (Catalog # MAB11727) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for S100A4 at approximately 11 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Detection of Mouse S100A4 by Simple Western^TM. Left: Simple Western lane view shows lysates of C2C12 mouse myoblast cell line, loaded at 0.2 mg/ml. A specific band was detected for S100A4 at approximately 9 kDa (as indicated) using both 1 µg/ml and 5 µg/ml of Rabbit Anti-Mouse S100A4 Monoclonal Antibody (Catalog # MAB11727) followed by HRP-conjugated Goat Anti-Rabbit Secondary Antibody (Catalog # 042-206). This experiment was conducted under reducing conditions and using the 2-40kDa separation system. Right: Simple Western electropherogram showing the same Rabbit Anti-Mouse S100A4 Monoclonal Antibody (Catalog # MAB11727) tested at 1 µg/ml (blue line) and 5 µg/ml (green line) in the C2C12 mouse myoblast cell line.