Mouse Semaphorin 4D/CD100 Antibody

Catalog #: MAB52351 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB52351
MAB52351-SP

Save 15% on Select RUO Reagents. See Details.

Detection of Semaphorin 4D/CD100 in Mouse Splenocytes by Flow Cytometry.

1 Image

Product Details

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Semaphorin 4D/CD100 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Semaphorin 4D/CD100 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Semaphorin 3A, 3B, 3C, 3D, 3E, 3F, 4A, 4B, 4C, 4D, 4G, 5A, 5B, 6A, 6B, 6C, 6D, or 7A is observed.

Source

Monoclonal Rat IgG_2B Clone # 547412

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Semaphorin 4D/CD100
Phe24-Thr657
Accession # NP_038688

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Flow Cytometry

2.5 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

View Larger

Detection of Semaphorin 4D/CD100 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Mouse Semaphorin 4D/CD100 Monoclonal Antibody (Catalog # MAB52351, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG F(ab')₂Secondary Antibody (Catalog # F0105B).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Semaphorin 4D/CD100

Semaphorin 4D (Sema4D/CD100, previously sem J, G or C-like 2) is a widely expressed 150 kDa type I transmembrane glycoprotein of the Class 4 Semaphorin family with activity in the immune and nervous systems. The 861 amino acid (aa) Sema4D contains a 710 aa extracellular domain (ECD) with a sema domain and an immunoglobulin-like domain. Within the ECD region used as an immunogen, mouse Sema4D shares 87% and 93% aa identity with human and rat Sema4D, respectively. A soluble, active 120 kDa form is produced proteolytically upon activation of B and T cells. Sema4D binds Plexin-B1, but also CD72 on B and dendritic cells. In developing neurons, Sema-4D assists in guidance. Sema4D is proangiogenic when expressed by tumor-associated macrophages.

Entrez Gene IDs

10507 (Human); 20354 (Mouse); 306790 (Rat)

Alternate Names

A8; BB18; CD100 antigen; CD100; CD100M-sema-G; COLL-4; FLJ33485; FLJ34282; FLJ39737; FLJ46484; immunoglobulin domain (Ig), transmembrane domain (TM) and shortcytoplasmic domain, 4D; MGC169138; MGC169141; M-sema-G; sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and shortcytoplasmic domain, (semaphorin) 4D; SEMA4D; SEMAJ; Semaphorin 4D; semaphorin-4D