Mouse Semaphorin 6A Alexa Fluor® 532-conjugated Antibody

Catalog #: FAB1615X Datasheet / COA / SDS

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FAB1615X-100UG

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Mouse Semaphorin 6A Alexa Fluor® 532-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Semaphorin 6A in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant human (rh) Semaphorin 6A is observed and no cross-reactivity with recombinant mouse Semaphorins 3C, 3

Source

Monoclonal Rat IgG2a Clone # 201932

Purification

Protein A or G purified

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse Semaphorin 6A
Gly19-Thr649
Accession # O35464

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 532 (Excitation= 534 nm, Emission= 553 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Semaphorin 6A

Semaphorin 6A (Sema6A) is a member of the class 6 semaphorin family of type I transmembrane proteins important in axon guidance during neuronal development. Class 6 semaphorins mediate bidirectional signaling, in part through interaction with plexin A1. In addition to neuronal expression, Semaphorin 6A is induced by IFN-gamma in Langerhans cells. Mature mouse and human Semaphorin 6A extracellular domains share 95% amino acid sequence identity.

Entrez Gene IDs

57556 (Human); 20358 (Mouse)

Alternate Names

KIAA1368HT018; sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6A; Sema VIA; SEMA; Sema6A; SEMA6A1; SEMA6A-1; Semaphorin 6A; semaphorin 6A-1; Semaphorin VIA; semaphorin-6A; semaphorin-6A-1; SEMAQ; VIA