Mouse Serpin A8/Angiotensinogen Alexa Fluor® 647-conjugated Antibody

Catalog #: FAB6966R Datasheet / COA / SDS

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FAB6966R-100UG

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Mouse Serpin A8/Angiotensinogen Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Serpin A8/Angiotensinogen in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) Serpin A8/Angiotensinogen, rhSerpin A4, recombinant mouse (rm) Serpin A1, A3N, A11, B8, C1, E1, E2, I1, or rmPED

Source

Monoclonal Rat IgG1 Clone # 754438

Purification

Protein A or G purified

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant mouse Serpin A8/Angiotensinogen
Asp25-Val477
Accession # P11859

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 647 (Excitation= 650 nm, Emission= 668 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Serpin A8/Angiotensinogen

Serpin A8 (serine proteinase inhibitor-clade A #8; also angiotensinogen/ANGT) is a secreted, 52-62 kDa glycoprotein member of the clade F-subfamily, serpin superfamily of protease inhibitors. It is expressed by neurons and hepatocytes, and undergoes extracellular cleavage by renin to create a ten amino acid (aa) peptide termed Ang/angiotensin I. This inactive peptide is further cleaved by ACE on the endothelial cell membrane to create bioactive Ang II. Ang II induces vascoconstriction and aldosterone release. Mature human Serpin A8 is 452 aa in length (aa 34-485). It contains Ang I (aa 34-43) that is cleaved to create Ang II (aa 34-41). Serpin A8 may circulate in a 200 kDa complex with major basic protein (MBP), or as part of a larger 300 kDa complex with MBP and complement C3dg. Over aa 34-485, human Serpin A8 shares 61% aa identity with mouse Serpin A8.

Entrez Gene IDs

183 (Human); 11606 (Mouse); 24179 (Rat)

Alternate Names

AGT; alpha-1 antiproteinase, antitrypsin; Angiotensin II; angiotensinogen (serpin peptidase inhibitor, clade A, member 8); Angiotensinogen; ANHU; Aogen; FLJ92595; FLJ97926; PAT; pre-angiotensinogen; serine (or cysteine) proteinase inhibitor; Serpin A8; SERPINA8angiotensin I