Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, Immunocytochemistry

Cited:

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all USP2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse USP2 isoform 2
Lys47-Met396
Accession # NP_932759

Specificity

Detects mouse USP2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, 100% cross-reactivity with recombinant human USP2 is observed. 

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse USP2 Antibody

USP2 antibody in NIH-3T3 Mouse Cell Line by Immunocytochemistry (ICC).

USP2 in NIH‑3T3 Mouse Cell Line.

USP2 was detected in immersion fixed NIH-3T3 mouse embryonic fibroblast cell line using Goat Anti-Mouse USP2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5804) at 0.3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Mouse USP2 antibody by Western Blot.

Detection of Mouse USP2 by Western Blot.

Western blot shows lysates of mouse liver tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse USP2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5804) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for USP2 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
USP2 antibody in Mouse Kidney by Immunohistochemistry (IHC-Fr).

USP2 in Mouse Kidney.

USP2 was detected in perfusion fixed frozen sections of mouse kidney using Goat Anti-Mouse USP2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5804) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in glandular epithelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Mouse USP2 Antibody

Application
Recommended Usage

Immunocytochemistry

0.3-25 µg/mL
Sample: Immersion fixed NIH-3T3 mouse embryonic fibroblast cell line

Immunohistochemistry

3-15 µg/mL
Sample: Perfusion fixed frozen sections of mouse kidney

Western Blot

1 µg/mL
Sample: Mouse liver tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: USP2

USP2 (Ubiquitin-specific-processing protease 2; also Ubiquitin carboxy-terminal hydrolase 2) is a cytosolic member of the peptidase C19 family of enzymes. It is widely expressed and serves to deubiquitinate select target substrates. USP2 effects are determined by its targets. In cells where it increases Mdm2, p53 is degraded and apoptosis is blocked. In cells where it increases Itch concentration, Flip activity is interrupted and apoptosis is promoted. USP2 is reported to form homo‑oligomers. There are at least two isoform variants. Each is approximately 70 kDa in size, with one showing a 266 aa, and another a 272 aa substitution for aa 1‑49. Over aa 47‑396, mouse USP2 shares 96% and 99% aa identity with human and rat USP2, respectively.

Long Name

Ubiquitin-specific Protease 2

Alternate Names

UBP41, USP9

Entrez Gene IDs

9099 (Human); 53376 (Mouse); 115771 (Rat)

Gene Symbol

USP2

UniProt

NP_932759

Additional USP2 Products

Product Documents for Mouse USP2 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse USP2 Antibody

For research use only

Related Research Areas

Deubiquitinating Enzymes (DUBs)

Citations for Mouse USP2 Antibody

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Protocols

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