Mouse Wnt-11 Antibody (310622)

R&D Systems | Catalog # MAB3746

R&D Systems
Discontinued Product
MAB3746 has been discontinued. View all Wnt-11 products.

Key Product Details

Species Reactivity

Mouse

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG1 Clone # 310622
View all Wnt-11 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse Wnt-11
Leu39-Ala79 and Ser225-Arg297
Accession # NP_033545

Specificity

Detects mouse Wnt-11 in direct ELISAs and Western blots. In these formats, this antibody shows no cross-reactivity with recombinant human Wnt-2, -7a, -7b, recombinant mouse Wnt-1, -3a, -4, -5a, -5b, -8a, -8b, or –9a. Western blotting with human cell extracts demonstrates that this antibody also detects human Wnt-11.

Clonality

Monoclonal

Host

Rat

Isotype

IgG1

Scientific Data Images for Mouse Wnt-11 Antibody (310622)

Detection of Mouse Wnt-11 antibody by Western Blot.

Detection of Mouse Wnt‑11 by Western Blot.

Western blot shows lysates of mouse embryo (13 d.p.c.) tissue. PVDF Membrane was probed with 2 µg/mL of Rat Anti-Mouse Wnt-11 Monoclonal Antibody (Catalog # MAB3746) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Wnt-11 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Wnt-11 antibody in Mouse Embryo by Immunohistochemistry (IHC-Fr).

Wnt‑11 in Mouse Embryo.

Wnt-11 was detected in immersion fixed frozen sections of mouse embryo (15 d.p.c.) using Rat Anti-Mouse Wnt-11 Monoclonal Antibody (Catalog # MAB3746) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of muscle cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Applications for Mouse Wnt-11 Antibody (310622)

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed frozen sections of mouse embryo (15 d.p.c.)

Western Blot

2 µg/mL
Sample: Mouse embryo (13 d.p.c.) tissue

Reviewed Applications

Read 2 reviews rated 4.5 using MAB3746 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Wnt-11

Wnt-11 belongs to the Wnt family of secreted, highly conserved, cysteine-rich signaling glcoproteins that are important in vertebrate development. Mouse Wnt-11a is expressed in early development and is required for cardiogenesis and branching morphogenesis of ureters. Wnt-11 signals by a non-canonical pathway and may repress canonical signaling by other Wnts. Mouse and human Wnt‑11 shares 96% identity over the sequences expressed in the immunogen.

Long Name

Wingless-type MMTV Integration Site Family, Member 11

Alternate Names

Wnt11

Entrez Gene IDs

7481 (Human); 22411 (Mouse)

Gene Symbol

WNT11

UniProt

NP_033545

Additional Wnt-11 Products

Product Documents for Mouse Wnt-11 Antibody (310622)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse Wnt-11 Antibody (310622)

For research use only

Related Research Areas

Wnt Ligands (Wnts)

Customer Reviews for Mouse Wnt-11 Antibody (310622) (2)

4.5 out of 5
2 Customer Ratings
5 Stars
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3 Stars
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1 Stars
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  • Mouse Wnt-11 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Colorectal cancer tissue
    Species: Mouse
    Verified Customer | Posted 12/13/2021
    Mouse Wnt-11 Antibody (310622) MAB3746
  • Mouse Wnt-11 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: E11.5 mouse embryo fixed in 4% PFA
    Species: Mouse
    Verified Customer | Posted 12/09/2020
    Staining was done on E11.5 mouse embryo heart sections (Right Ventricle) (4% PFA fixed). Concentration used - 8ug/mL.
    Mouse Wnt-11 Antibody (310622) MAB3746

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