MSH6 Antibody (MSVA-906R)

Novus Biologicals | Catalog # NBP3-57234

Recombinant Monoclonal Antibody
Novus Biologicals

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # MSVA-906R
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Product Specifications

Immunogen

Recombinant fragment (around aa 1-100) of human MSH6 protein (exact sequence is proprietary) Swissprot P52701

Localization

Nuclear

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody is highly specific and optimized for use in Immunohistochemistry.

It has been validated on TMAs covering 76 normal tissues and numerous cancer tissues.

The antibody concentration is optimized for dilution within dilution range using commercially available antibody diluent for immunohistochemistry.

Positive Control
Postive control:Tonsil: Virtually all mantle zone B-cells must show an at least weak to moderate nuclear staining. A moderate to strong nuclear staining must be seen in the germinal centre B-cells..

Negative Control
Negitve control:Colon adenocarcinoma with previously documented loss of MSH6 expression: Nuclear staining should be absent in cancer cells, while a nuclear staining must be seen in stromal and inflammatory cells.

Storage
Antibody can be stored at 4degC. For extended storage, aliquot and store at -20degC. Avoid repeated freeze-thaw cycles.

Scientific Data Images for MSH6 Antibody (MSVA-906R)

MSH6 Antibody (MSVA-906R)

Immunohistochemistry-Paraffin:MSH6 Antibody (MSVA-906R)NBP3-57234

Colon Colorectal adenocarcinoma showing strong MSH6 positivity in cancer cells and moderate staining in stromal cells
MSH6 Antibody (MSVA-906R)

Immunohistochemistry-Paraffin:MSH6 Antibody (MSVA-906R)NBP3-57234

Colon descendens mucosa
MSH6 Antibody (MSVA-906R)

Immunohistochemistry-Paraffin:MSH6 Antibody (MSVA-906R)NBP3-57234

Colon Colorectal adenocarcinoma with strong MSH6 immunostaining in stromal and inflammatory cells but absent MSH6 staining in cancer cells

Applications for MSH6 Antibody (MSVA-906R)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:75 1:150
Application Notes
Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:100 at 37C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS

Preservative

<0.1% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: MSH6

MSH6 is a mismatch-repair protein. It plays a role in tumor suppression. Genomic mutations in MSH6 predispose to the hereditary non-polyposis colorectal cancer (HNPCC) syndrome.

Long Name

mutS homolog 6

Alternate Names

GTBP, GTMBP, HNPCC5, HSAP, LYNCH5, MMRCS3, MSH-6, p160

Gene Symbol

MSH6

Additional MSH6 Products

Product Documents for MSH6 Antibody (MSVA-906R)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for MSH6 Antibody (MSVA-906R)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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