MTAP Antibody (MSVA-741R)

Novus Biologicals | Catalog # NBP3-57265

Recombinant Monoclonal Antibody
Novus Biologicals

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # MSVA-741R
View all MTAP Primary Antibodies »

Product Specifications

Immunogen

Recombinant human MTAP protein fragment (aa95-197) (exact sequence is proprietary) Swissprot Q13126

Localization

Nuclear

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody is highly specific and optimized for use in Immunohistochemistry.

It has been validated on TMAs covering 76 normal tissues and numerous cancer tissues.

The antibody concentration is optimized for dilution within dilution range using commercially available antibody diluent for immunohistochemistry.

Positive Control
Postive control:Ovary: At least a moderate, nuclear and/or cytoplasmic MTAP staining should be seen in ovarian stroma cells..

Negative Control
Negitve control:Bladder cancer with homozygous 9p deletion: MTAP staining should be absent in cells from urothelial tumors with homozygous 9p deletion.

Storage
Antibody can be stored at 4degC. For extended storage, aliquot and store at -20degC. Avoid repeated freeze-thaw cycles.

Scientific Data Images for MTAP Antibody (MSVA-741R)

MTAP Antibody (MSVA-741R)

Immunohistochemistry-Paraffin:MTAP Antibody (MSVA-741R)NBP3-57265

Non invasive urothelial carcinoma pTa with complete absence of MTAP staining in all tumor cells. MTAP positive stroma cells serve as an internal control
MTAP Antibody (MSVA-741R)

Immunohistochemistry-Paraffin:MTAP Antibody (MSVA-741R)NBP3-57265

Moderate predominantly cytoplasmic MTAP staining of ovarian stroma cells
MTAP Antibody (MSVA-741R)

Immunohistochemistry-Paraffin:MTAP Antibody (MSVA-741R)NBP3-57265

Urothelium with intense cytoplasmic and nuclear MTAP staining of all epithelial cells

Applications for MTAP Antibody (MSVA-741R)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:100 1:200
Application Notes
Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS

Preservative

<0.1% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: MTAP

MTAP encodes an enzyme that plays a major role in polyamine metabolism and is important for the salvage of both adenine and methionine. The encoded enzyme is deficient in many cancers because this gene and the tumor suppressor p16 gene are co-deleted. Multiple alternatively spliced transcript variants have been described for this gene, but their full-length natures remain unknown.

Long Name

5'-Methylthioadenosine Phosphorylase

Alternate Names

BDMF, C86fus, DMSFH, DMSMFH, HEL-249, LGMBF, MTAPase

Gene Symbol

MTAP

Additional MTAP Products

Product Documents for MTAP Antibody (MSVA-741R)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for MTAP Antibody (MSVA-741R)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Sulfation Regulators

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Protocols

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FAQs

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