mTOR Complex 2 Antibody Pack
Novus Biologicals | Catalog # NBP3-20209
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Key Product Details
Species
Human, Mouse
Applications
Immunohistochemistry, Western Blot
Product Summary for mTOR Complex 2 Antibody Pack
This pack contains 1 vial each of: AF15371-SP (25 ug), AF15371-SP (0.025 ml), NBP1-51645SS (25 ug), AF4004-SP (25 ug), MAB8168-SP (25 ug), AF5309-SP (0.025 ml), NBP1-49674SS (1 mL), HAF008 (1 mL), HAF007 (1 mL).
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Product Specifications
Application Notes
See individual datasheets of components for their validated applications
Reactivity Notes
See individual datasheets of components for their validated species
Scientific Data Images for mTOR Complex 2 Antibody Pack
Detection of Rictor in HeLa by Flow Cytometry.
An intracellular stain was performed on HeLa cells with Rictor Antibody (7B3) NBP1-51645PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.
Detection of G beta L (GBL) in Human Prostate by Immunohistochemistry (IHC-P).
G beta L was detected in immersion fixed paraffin-embedded sections of human prostate using Goat Anti-Human/Mouse/Rat G beta L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4004) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Sin1/MAPKAP1 in Human Heart and Kidney Tissue by Western Blot.
Western blot shows lysates of human heart tissue and human kidney tissue. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human Sin1/MAPKAP1 Monoclonal Antibody (Catalog # MAB8168) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for Sin1/MAPKAP1 at approximately 59-63 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of PRR5 in HepG2, Jurkat and MCF-7 lysate by Western Blot.
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, Jurkat human acute T cell leukemia cell line, and MCF-7 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Human PRR5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5309) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for PRR5 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Detection of DEPTOR/DEPDC6 in Human and Mouse Liver by Western Blot.
Western blot shows lysates of (A) human liver and (B) mouse liver. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human DEPTOR/DEPDC6 Antigen Affinity-purified Polyclonal Antibody (Catalog # NBP1-49674) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody. This experiment was conducted under reducing conditions.Kit Contents for mTOR Complex 2 Antibody Pack
- AF15371-SP
- AF4004-SP
- AF5309-SP
- HAF007: Goat anti-Mouse IgG Secondary Antibody [HRP]
- HAF008: Goat anti-Rabbit IgG Secondary Antibody [HRP]
- MAB8168-SP
- NBP1-49674SS
- NBP1-51645SS
- HAF017: Rabbit anti-Goat IgG Secondary Antibody [HRP]
Formulation, Preparation, and Storage
Concentration
Concentration of individual antibodies may be found on the vial label. If unlisted please contact technical services.
Storage
Storage is content dependent.
Product Documents for mTOR Complex 2 Antibody Pack
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for mTOR Complex 2 Antibody Pack
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Packs are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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