MTSS1 Antibody - BSA Free

Western Blot: MTSS1 Antibody - BSA Free [NBP2-24716] -

Expression of Mtss1 induces I-BAR domain-dependent morphological changes in COS7 cells, generating protrusions.(A) Western blot images showing that weakly expression of endogenous Mtss1 was not altered by overexpression of Plexin-D1 with or without Sema3E in COS7 cells. Asterisk indicates nonspecific band. (B) Quantification of the band intensity in (A). Error bars, mean +/- SEM; ns p>0.05 by two-way ANOVA with Bonferroni’s post hoc correction for multiple comparisons; n = 3. (C) Schematics describing the full-length construct of Mtss1-myc and its deletion mutant constructs (Mtss1 delta I-BAR-myc, Mtss1 delta WH2-myc, and I-BAR-myc). (D) Immunocytochemistry images taken after overexpression of each construct. Constructs show the I-BAR domain leading to diverse cell protrusion morphologies. Some of the protrusions were excessively spiked or thin and long (arrowheads). Overexpression of the I-BAR domain only (I-BAR-myc) can induce extreme protrusion structures. Scale bar, 20 μm.Figure 3—figure supplement 1—source data 1.Western blots shown in Figure 3—figure supplement 1A.Western blots shown in Figure 3—figure supplement 1A. Image collected and cropped by CiteAb from the following open publication (https://elifesciences.org/articles/96891), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: MTSS1 Antibody - BSA Free [NBP2-24716] -

The absence of Mtss1 does not affect medium spiny neuron (MSN) survival, dendritic arborization, and Plexin-D1 expression during striatonigral pathway development.(A) Immunohistochemistry staining for cleaved caspase 3 (CC3) in the striatum of wild-type (WT) or Mtss1 conditional knockout (cKO) mice. The white dotted boxes on the left images are shown in the inset images on the right at a better resolution. Scale bar, 25 μm. (B) Quantification of cell death by the number of CC3-positive cells in a 1 mm2 area covering the dorsal part of the striatum in WT or Mtss1 cKO mice. Error bars, mean +/- SEM; ns p>0.05 by Mann‒Whitney test; WT, n = 20; Mtss1 cKO mice, n = 20 (five sections/mouse). (C) Representative images of Golgi staining at low (top panels) and high (bottom panels) magnification. Scale bars, 100 μm. Sholl analysis of dendritic morphology (D) and dendritic length (E) performed by using Neurolucida360 in 3D analysis. Error bars, mean +/- SEM; ns p>0.05 by Student’s t-test; WT, n = 12, and Mtss1 cKO mice, n = 15, from three mice. (F, G) Western blot images and quantification of Plexin-D1 expression in the striatum of WT or Mtss1 cKO mice at P5. Error bars, mean +/- SEM; *p<0.05 by Student’s t-test; WT mice, n = 3, and Mtss1 cKO mice, n = 4. (H) Plexin-D1 expression in MSNs obtained from the striatum of WT or Mtss1 cKO mice at P0 and measured at DIV6 in culture. (I) Quantification of the western blots shown in (H). Error bars, mean +/- SEM; *p<0.05, by Student’s t-test; n = 3 for WT, n = 3 for KO in three independent experiments.Figure 7—figure supplement 3—source data 1.Raw uncropped western blot & gel images.Western blots shown in Figure 7—figure supplement 3F and H.Raw uncropped western blot & gel images.Western blots shown in Figure 7—figure supplement 3F and H. Image collected and cropped by CiteAb from the following open publication (https://elifesciences.org/articles/96891), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: MTSS1 Antibody - BSA Free [NBP2-24716] -

The absence of Mtss1 does not affect medium spiny neuron (MSN) survival, dendritic arborization, and Plexin-D1 expression during striatonigral pathway development.(A) Immunohistochemistry staining for cleaved caspase 3 (CC3) in the striatum of wild-type (WT) or Mtss1 conditional knockout (cKO) mice. The white dotted boxes on the left images are shown in the inset images on the right at a better resolution. Scale bar, 25 μm. (B) Quantification of cell death by the number of CC3-positive cells in a 1 mm2 area covering the dorsal part of the striatum in WT or Mtss1 cKO mice. Error bars, mean +/- SEM; ns p>0.05 by Mann‒Whitney test; WT, n = 20; Mtss1 cKO mice, n = 20 (five sections/mouse). (C) Representative images of Golgi staining at low (top panels) and high (bottom panels) magnification. Scale bars, 100 μm. Sholl analysis of dendritic morphology (D) and dendritic length (E) performed by using Neurolucida360 in 3D analysis. Error bars, mean +/- SEM; ns p>0.05 by Student’s t-test; WT, n = 12, and Mtss1 cKO mice, n = 15, from three mice. (F, G) Western blot images and quantification of Plexin-D1 expression in the striatum of WT or Mtss1 cKO mice at P5. Error bars, mean +/- SEM; *p<0.05 by Student’s t-test; WT mice, n = 3, and Mtss1 cKO mice, n = 4. (H) Plexin-D1 expression in MSNs obtained from the striatum of WT or Mtss1 cKO mice at P0 and measured at DIV6 in culture. (I) Quantification of the western blots shown in (H). Error bars, mean +/- SEM; *p<0.05, by Student’s t-test; n = 3 for WT, n = 3 for KO in three independent experiments.Figure 7—figure supplement 3—source data 1.Raw uncropped western blot & gel images.Western blots shown in Figure 7—figure supplement 3F and H.Raw uncropped western blot & gel images.Western blots shown in Figure 7—figure supplement 3F and H. Image collected and cropped by CiteAb from the following open publication (https://elifesciences.org/articles/96891), licensed under a CC-BY license. Not internally tested by Novus Biologicals.