MUC6 Antibody (MSVA-806R) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-57314

Recombinant Monoclonal Antibody
Novus Biologicals

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # MSVA-806R

Format

Azide and BSA Free
View all MUC6 Primary Antibodies »

Product Specifications

Immunogen

Recombinant full-length human MUC6 protein Swissprot Q6W4X9

Localization

Cytoplasmic

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody is highly specific and optimized for use in Immunohistochemistry.

It has been validated on TMAs covering 76 normal tissues and numerous cancer tissues.

Positive Control
Postive control:Stomach: Mucous secreting glands should be strongly positive.

Negative Control
Negitve control:Stomach: Surface epithelium should be completely negative.

Applications for MUC6 Antibody (MSVA-806R) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MUC6

Mucin is a high M.W. (>1,000 kDa) glycoprotein, expressed by mucus cells of the gastric epithelium and by goblet cells of the fetal, precancerous and cancerous colon, but not by those of the normal colon. It also appears in other epithelial tissues, which are embryologically derived from the foregut (epigastric and bronchial epithelium) and in M ller ducts (mucus cells of the endocervix and urethral epithelium near the prostatic utriculus).

Alternate Names

gastric mucin, Gastric mucin-6, MUC-6, mucin 6, gastric, mucin 6, oligomeric mucus/gel-forming, mucin-6

Gene Symbol

MUC6

Additional MUC6 Products

Product Documents for MUC6 Antibody (MSVA-806R) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for MUC6 Antibody (MSVA-806R) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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