Myosin Phosphatase Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-68929

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (93%), Rat (96%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Myosin Phosphatase Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: DILGYLEELQKKQNLLHSEKRDKKSPLIESTANMDNNQSQKTFKNKETLIIEPEKNASRIESLEQEKVDEEEEGKKDESSCS

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Myosin Phosphatase Antibody - BSA Free

Western Blot: Myosin Phosphatase Antibody [NBP2-68929]

Western Blot: Myosin Phosphatase Antibody [NBP2-68929]

Western Blot: Myosin Phosphatase Antibody [NBP2-68929] - Analysis in human cell line RT-4 and human cell line U-251 MG.
Immunohistochemistry-Paraffin: Myosin Phosphatase Antibody [NBP2-68929]

Immunohistochemistry-Paraffin: Myosin Phosphatase Antibody [NBP2-68929]

Immunohistochemistry-Paraffin: Myosin Phosphatase Antibody [NBP2-68929] - Stainng of human placenta shows moderate cytoplasmic positivity in trophoblastic cells.
Immunohistochemistry-Paraffin: Myosin Phosphatase Antibody [NBP2-68929]

Immunohistochemistry-Paraffin: Myosin Phosphatase Antibody [NBP2-68929]

Immunohistochemistry-Paraffin: Myosin Phosphatase Antibody [NBP2-68929] - Staining of human liver shows low positivity in hepatocytes as expected.
Immunohistochemistry-Paraffin: Myosin Phosphatase Antibody [NBP2-68929]

Immunohistochemistry-Paraffin: Myosin Phosphatase Antibody [NBP2-68929]

Immunohistochemistry-Paraffin: Myosin Phosphatase Antibody [NBP2-68929] - Staining of human urinary bladder shows strong cytoplasmic positivity in urothelial cells.
Immunohistochemistry-Paraffin: Myosin Phosphatase Antibody [NBP2-68929]

Immunohistochemistry-Paraffin: Myosin Phosphatase Antibody [NBP2-68929]

Immunohistochemistry-Paraffin: Myosin Phosphatase Antibody [NBP2-68929] - Staining of human prostate shows weak to moderate cytoplasmic positivity in glanular and smooth muscle cells.

Applications for Myosin Phosphatase Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
Recommended conditions for IHC,Retrieval method: HIER pH6

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Myosin Phosphatase

The major protein phosphatase-1 (PP1) is composed of 3 subunits with apparent molecular mass of 110-130, 37 & 20 kDa. The 110-130 kDa component act as a regulatory subunit known as myosine binding (MBS) or myosine phosphatae targeting subunit (MYPT). The N-terminal portion of MYPT binds to both PP1c-delta and phosphorylated myosin, thereby increasing myosin phosphatase activity. Two isoform of MYPT have been identified (MYPT1 & MYPT2) and share 61% sequence identity. While MYPT1 is widely distributed in human tissues, MYPT2 is mostly detected in brain and heart.

Alternate Names

MBSmyosin phosphatase, target subunit 1, MGC133042, Myosin phosphatase target subunit 1, Myosin phosphatase-targeting subunit 1, MYPT1protein phosphatase 1 regulatory subunit 12A, protein phosphatase 1, regulatory (inhibitor) subunit 12A, Protein phosphatase myosin-binding subunit

Gene Symbol

PPP1R12A

Additional Myosin Phosphatase Products

Product Documents for Myosin Phosphatase Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Myosin Phosphatase Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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