N myc interactor Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-90374

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation-exo-Seq

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all N myc interactor Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: KINVTEIPDTLREDQMRDKLELSFSKSRNGGGEVDRVDYDRQSGSAVITFVEIGVADKILKKKEYPLYINQTCHRVTVSPYTEIHLKKYQIFSGTSKRTVLLTGMEGIQMDEEIVEDLINIHFQRAKNGGGEVDVVKCSLGQPH

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for N myc interactor Antibody - BSA Free

N myc interactor Antibody - BSA Free Immunocytochemistry/ Immunofluorescence: N myc interactor Antibody - BSA Free [NBP1-90374]

Immunocytochemistry/ Immunofluorescence: N myc interactor Antibody - BSA Free [NBP1-90374]

Staining of human cell line A-431 shows localization to nucleoplasm & cytosol.
N myc interactor Antibody - BSA Free Immunohistochemistry: N myc interactor Antibody - BSA Free [NBP1-90374]

Immunohistochemistry: N myc interactor Antibody - BSA Free [NBP1-90374]

Staining of human tonsil shows moderate cytoplasmic positivity in non-germinal center cells.
N myc interactor Antibody - BSA Free Immunohistochemistry: N myc interactor Antibody - BSA Free [NBP1-90374]

Immunohistochemistry: N myc interactor Antibody - BSA Free [NBP1-90374]

Staining of human spleen shows moderate cytoplasmic positivity in cells in white pulp.
N myc interactor Antibody - BSA Free Immunohistochemistry: N myc interactor Antibody - BSA Free [NBP1-90374]

Immunohistochemistry: N myc interactor Antibody - BSA Free [NBP1-90374]

Staining of human cerebellum shows no positivity in Purkinje cells as expected.
N myc interactor Antibody - BSA Free Immunohistochemistry: N myc interactor Antibody - BSA Free [NBP1-90374]

Immunohistochemistry: N myc interactor Antibody - BSA Free [NBP1-90374]

Staining of human colon shows strong cytoplasmic positivity in glandular cells.
N myc interactor Antibody - BSA Free Chromatin Immunoprecipitation-exo-Seq: N myc interactor Antibody - BSA Free [NBP1-90374]

Chromatin Immunoprecipitation-exo-Seq: N myc interactor Antibody - BSA Free [NBP1-90374]

ChIP-Exo-Seq composite graph for Anti-NMI (NBP1-90374) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

Applications for N myc interactor Antibody - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation-exo-Seq

1-10ug per reaction

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: N myc interactor

NMYC interactor (NMI) encodes a protein that interacts with NMYC and CMYC (two members of the oncogene Myc family), and other transcription factors containing a Zip, HLH, or HLH-Zip motif. The NMI protein also interacts with all STATs except STAT2 and augments STAT-mediated transcription in response to cytokines IL2 and IFN-gamma. The NMI mRNA has low expression levels in all human fetal and adult tissues tested except brain and has high expression in cancer cell line-myeloid leukemias.

Alternate Names

Nmi, N-myc (and STAT) interactor, N-myc and STAT interactor, N-myc interactor, N-myc-interactor

Gene Symbol

NMI

Additional N myc interactor Products

Product Documents for N myc interactor Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for N myc interactor Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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