NDUFS3 Antibody (2R6F5)

Name: NDUFS3 Antibody (2R6F5)
Brand: Novus Biologicals
SKU: NBP3-16287
Price: 389 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-16287

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Product Documents

Recombinant Monoclonal Antibody

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2R6F5 expressed in HEK293

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Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 165-264 of human NDUFS3 (O75489). WYEREIWDMFGVFFANHPDLRRILTDYGFEGHPFRKDFPLSGYVELRYDDEVKRVVAEPVELAQEFRKFDLNSPWEAFPVYRQPPESLKLEAGDKKPDAK

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NDUFS3 Antibody (2R6F5)

Western Blot: NDUFS3 Antibody (2R6F5) [NBP3-16287]

Western Blot: NDUFS3 Antibody (2R6F5) [NBP3-16287] - Western blot analysis of extracts of various cell lines, using NDUFS3 Rabbit mAb (NBP3-16287) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunohistochemistry-Paraffin: NDUFS3 Antibody (2R6F5) [NBP3-16287]

Immunohistochemistry-Paraffin: NDUFS3 Antibody (2R6F5) [NBP3-16287] - Immunohistochemistry of paraffin-embedded rat liver using NDUFS3 Rabbit mAb (NBP3-16287) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Applications for NDUFS3 Antibody (2R6F5)

Application

Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: NDUFS3

The multisubunit NADH:ubiquinone oxidoreductase (complex I) is the first enzyme complex in the electron transport chain of mitochondria. The iron-sulfur protein (IP) fraction of complex I is made up of 7 subunits. See NDUFS1 (MIM 157655).[supplied by OMIM]

Alternate Names

CI-30, CI-30kD, complex I 30kDa subunit, Complex I-30kD, EC 1.6.5.3, EC 1.6.99.3, EC 1.6.99.5, NADH dehydrogenase (ubiquinone) Fe-S protein 3 (30kD) (NADH-coenzyme Qreductase), NADH dehydrogenase (ubiquinone) Fe-S protein 3, 30kDa (NADH-coenzyme Qreductase), NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial, NADH dehydrogenase-ubiquinone 30 kDa subunit, NADH-ubiquinone oxidoreductase 30 kDa subunit

Gene Symbol

NDUFS3

Additional NDUFS3 Products

Product Documents for NDUFS3 Antibody (2R6F5)

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Certificate of Analysis

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Product Specific Notices for NDUFS3 Antibody (2R6F5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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