Nesprin 2 Antibody

Novus Biologicals | Catalog # NBP2-42886

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (98%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all Nesprin 2 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of mouse Nesprin 2. The exact sequence is proprietary.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

783 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Nesprin 2 Antibody

Immunohistochemistry-Paraffin: Nesprin 2 Antibody [NBP2-42886]

Immunohistochemistry-Paraffin: Nesprin 2 Antibody [NBP2-42886]

Immunohistochemistry-Paraffin: Nesprin 2 Antibody [NBP2-42886] - Rat brain. Nesprin 2 stained by Nesprin 2 antibody [C3], C-term diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Nesprin 2 Antibody

Immunocytochemistry/ Immunofluorescence: Nesprin 2 Antibody [NBP2-42886] -

Nesprin 2 antibody [C3], C-term detects Nesprin 2 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Nesprin 2 stained by Nesprin 2 antibody [C3], C-term (NBP2-42886) diluted at 1:2000.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.
Nesprin 2 Antibody

Western Blot: Nesprin 2 Antibody [NBP2-42886] -

Various tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Nesprin 2 antibody [C3], C-term (NBP2-42886) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Nesprin 2 Antibody

Immunohistochemistry-Frozen: Nesprin 2 Antibody [NBP2-42886] -

Nesprin 2 antibody [C3], C-term detects Nesprin 2 protein expression by immunohistochemical analysis.
Sample: Frozen sectioned adult mouse retina.
Green: Nesprin 2 protein stained by Nesprin 2 antibody [C3], C-term (NBP2-42886) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:250.
Blue: Fluoroshield with DAPI.
Nesprin 2 Antibody

Immunocytochemistry/ Immunofluorescence: Nesprin 2 Antibody [NBP2-42886] -

Syne2 antibody [C3], C-term detects Syne2 protein at nucleus by immunofluorescent analysis.
Sample: H1299 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Syne2 protein stained by Syne2 antibody [C3], C-term (NBP2-42886) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 um.
Nesprin 2 Antibody

Immunohistochemistry-Paraffin: Nesprin 2 Antibody [NBP2-42886] -

Nesprin 2 antibody [C3], C-term detects Nesprin 2 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
Nesprin 2 stained by Nesprin 2 antibody [C3], C-term (NBP2-42886) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Applications for Nesprin 2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

Assay dependent

Immunohistochemistry-Frozen

1:100-1:1000

Immunohistochemistry-Paraffin

Assay dependent

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Nesprin 2

SYNE2 - spectrin repeat containing, nuclear envelope 2

Alternate Names

DKFZP434H2235, DKFZp686E01115, DKFZp686H1931, EDMD5, FLJ46790, KIAA1011FLJ11014, nesprin 2, nesprin-2, NUAFLJ43727, NUANCEFLJ45710, Nuclear envelope spectrin repeat protein 2, Nucleus and actin connecting element protein, polytrophin, Protein NUANCE, spectrin repeat containing, nuclear envelope 2, Synaptic nuclear envelope protein 2, synaptic nuclei expressed gene 2, SYNE-2, TROPH

Gene Symbol

SYNE2

Additional Nesprin 2 Products

Product Documents for Nesprin 2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Nesprin 2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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