NF-L Antibody (CL4729) - BSA Free

Novus Biologicals | Catalog # NBP2-59061

Novus Biologicals

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL4729

Format

BSA Free
View all NF-L Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from P07196, with the exact immunogen sequence remaining proprietary.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

Novus Biologicals Mouse NF-L Antibody (CL4729) - BSA Free (NBP2-59061) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for NF-L Antibody (CL4729) - BSA Free

Western Blot: NF-L Antibody (CL4729) [NBP2-59061]

Western Blot: NF-L Antibody (CL4729) [NBP2-59061]

Western Blot: NF-L Antibody (CL4729) [NBP2-59061] - Analysis in human cerebral cortex tissue.
Immunocytochemistry/ Immunofluorescence: NF-L Antibody (CL4729) [NBP2-59061]

Immunocytochemistry/ Immunofluorescence: NF-L Antibody (CL4729) [NBP2-59061]

Immunocytochemistry/Immunofluorescence: NF-L Antibody (CL4729) [NBP2-59061] - Staining of PC-3 cells using the Anti-NEFL monoclonal antibody, showing specific staining in intermediate filaments in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available). Antibody staining is shown in green.
Immunohistochemistry-Paraffin: NF-L Antibody (CL4729) [NBP2-59061]

Immunohistochemistry-Paraffin: NF-L Antibody (CL4729) [NBP2-59061]

Immunohistochemistry-Paraffin: NF-L Antibody (CL4729) [NBP2-59061] - NF-L Antibody [NBP2-59061] - Staining of human cerebral cortex shows strong positivity in neural fibers.
Immunohistochemistry: NF-L Antibody (CL4729) [NBP2-59061]

Immunohistochemistry: NF-L Antibody (CL4729) [NBP2-59061]

Immunohistochemistry: NF-L Antibody (CL4729) [NBP2-59061] - NF-L Antibody [NBP2-59061] - Staining of rat cerebral cortex shows strong immunoreactivity in neuronal cell bodies and processes.
Immunohistochemistry: NF-L Antibody (CL4729) [NBP2-59061]

Immunohistochemistry: NF-L Antibody (CL4729) [NBP2-59061]

Immunohistochemistry: NF-L Antibody (CL4729) [NBP2-59061] - NF-L Antibody [NBP2-59061] - Staining of mouse cerebral cortex shows strong immunoreactivity in neuronal cell bodies and processes.
Immunohistochemistry-Paraffin: NF-L Antibody (CL4729) [NBP2-59061]

Immunohistochemistry-Paraffin: NF-L Antibody (CL4729) [NBP2-59061]

Immunohistochemistry-Paraffin: NF-L Antibody (CL4729) [NBP2-59061] - Staining of human cerebellum shows strong cytoplasmic immunoreactivity in cell bodies and proximal dendrites of Purkinje cells.
Immunohistochemistry: NF-L Antibody (CL4729) [NBP2-59061]

Immunohistochemistry: NF-L Antibody (CL4729) [NBP2-59061]

Immunohistochemistry: NF-L Antibody (CL4729) [NBP2-59061] - NF-L Antibody [NBP2-59061] - Staining of rat brainstem shows strong immunoreactivity in neuronal cell bodies.
Immunohistochemistry: NF-L Antibody (CL4729) [NBP2-59061]

Immunohistochemistry: NF-L Antibody (CL4729) [NBP2-59061]

Immunohistochemistry: NF-L Antibody (CL4729) [NBP2-59061] - NF-L Antibody [NBP2-59061] - Staining of mouse striatum shows strong positivity in neural fibers.
Immunohistochemistry-Paraffin: NF-L Antibody (CL4729) [NBP2-59061]

Immunohistochemistry-Paraffin: NF-L Antibody (CL4729) [NBP2-59061]

Immunohistochemistry-Paraffin: NF-L Antibody (CL4729) [NBP2-59061] - Staining of human liver shows no positivity as expected (negative control).

Applications for NF-L Antibody (CL4729) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:10000 - 1:20000

Immunohistochemistry-Paraffin

1:10000 - 1:20000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation/Permeabilization: PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NF-L

Neurofilaments are 10nm intermediate filament proteins located in vertebrate neurons, which regulate axonal diameter. They are composed predominantly of the three major neurofilament proteins: NF-Light, NF-Medium and NF-Heavy. NF-L is the light or low molecular weight polypeptide, and it runs on SDS-PAGE gels at about 68kDa, with some size variation in across species.

Antibodies to NF-L may be used to identify NF-L in neurons and to study neuronal processes in tissue sections and/or culture. NF-L antibodies can also be used to study microfilament accumulations seen in many neurological diseases, such as Lou Geri's disease or Alzheimer's disease.

Long Name

Neurofilament Protein, Light Chain

Alternate Names

CMT2E, NEFL, NF68, NFL

Gene Symbol

NEFL

Additional NF-L Products

Product Documents for NF-L Antibody (CL4729) - BSA Free

Certificate of Analysis

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Product Specific Notices for NF-L Antibody (CL4729) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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