NF-L Antibody (DA2) - BSA Free

Novus Biologicals | Catalog # NB300-132

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Avian, Bovine, Mammal

Cited:

Human, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Simple Western

Cited:

Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # DA2

Format

BSA Free
View all NF-L Primary Antibodies »

Product Specifications

Immunogen

Native Neurofilament Light protein purified from pig spinal cord [UniProt# P02547]

Epitope

The epitope for this antibody is not in the N-terminus; however exactly where it is located is not known. It is thought to be somewhere within the central alpha helical rod region of the molecule.

Reactivity Notes

Bovine reactivity reported in scientific literature (PMID: 25819401).

Localization

Cytoplasmic

Marker

Neuronal Marker

Specificity

Specifically recognizes the light neurofilament subunit or NF-L (~68 kDa).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for NF-L Antibody (DA2) - BSA Free

Western Blot: NF-L Antibody (DA2) [NB300-132]

Western Blot: NF-L Antibody (DA2) [NB300-132]

Western Blot: NF-L Antibody (DA2) [NB300-132] - Rat spinal cord homogenate showing the major intermediate filament proteins of the nervous system (lane 1). The remaining lanes show blots of this material stained with various antibodies including NB300-132 (lane 4).
Immunocytochemistry/ Immunofluorescence: NF-L Antibody (DA2) [NB300-132]

Immunocytochemistry/ Immunofluorescence: NF-L Antibody (DA2) [NB300-132]

Immunocytochemistry/Immunofluorescence: NF-L Antibody (DA2) [NB300-132] - Staining of neurons in culture using NF-L monoclonal DA2 antibody (green) imaged with a 40X lens. Nuclei are stained blue. Photo Courtesy of Dr. Gerry Shaw.
Simple Western: NF-L Antibody (DA2) [NB300-132]

Simple Western: NF-L Antibody (DA2) [NB300-132]

Simple Western: NF-L Antibody (DA2) [NB300-132] - Image shows a specific band for NF-L in 0.5 mg/mL of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Applications for NF-L Antibody (DA2) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100 - 1:500

Immunohistochemistry

1:250

Immunohistochemistry-Paraffin

reported in scientific literature (PMID 25565998)

Simple Western

1:50

Western Blot

1:5000 - 1:10000
Application Notes
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in Human Brain lysate 0.5 mg/mL, separated by Size, antibody dilution of 1:50, apparent MW was 81 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Ascites

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NF-L

Neuronal intermediate filaments or neurofilaments are found specifically in neurons where they play a key role in maintaining neuronal morphology as well as in regenerating myelinated axons. Neurofilaments contain three subunits: a light polypeptide (NEFL/NFL), a medium polypeptide (NEFM/NFM), and a heavy polypeptide (NEFH/NFH), with molecular weights of 68, 160, and 212 kD respectively. 68kD Neurofilament Light (NEFL) is the most abundant of three neurofilament proteins and, like other nonepithelial intermediate filament proteins, it can form homopolymeric 10-nm filaments. NEFL forms the backbone of neurofilament and perturbations in its expression leads to motor neuron diseases, such as Charcot-Marie-Tooth disease, type 2E (CMT2E). In addition to its influence on the nervous system, NEFL has been shown to act as a tumor suppressor and has been linked to carcinogenesis/metastasis of several malignancues including breast, prostate, lung, colon, and bladder cancer.

Long Name

Neurofilament Protein, Light Chain

Alternate Names

CMT2E, NEFL, NF68, NFL

Entrez Gene IDs

4747 (Human)

Gene Symbol

NEFL

Additional NF-L Products

Product Documents for NF-L Antibody (DA2) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for NF-L Antibody (DA2) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for NF-L Antibody (DA2) - BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for NF-L Antibody (DA2) - BSA Free

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  • Q: Can you please tell me if the 68 kDa NF-L antibody (DA2) epitope is in the tail domain or the rod domain of the protein?

    A: The epitope for this antibody is not in the N-terminus; however, exactly where it is located is not known. It is thought to be somewhere within the central alpha helical rod region of the molecule.

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